G4WJD3 · COLC_YERPU

Function

function

Involved in the biosynthesis of the L-colitose (3,6-dideoxyl-L-xylo-hexose) present in the O-antigen region of lipopolysaccharides (LPS) where it serves as antigenic determinant and are vital for bacterial defense and survival. Catalyzes the two-step NADP-dependent conversion of GDP-4-keto-3,6-dideoxy-D-mannose to GDP-L-colitose. ColC is a bifunctional enzyme catalyzing the C-5 epimerization of GDP-4-keto-3,6-dideoxy-D-mannose and the subsequent C-4 keto reduction of the resulting L-epimer to give GDP-L-colitose. It can use both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
75 μMGDP-4-keto-3,6-dideoxy-D-mannose7.537with NADP
139 μMGDP-4-keto-3,6-dideoxy-D-mannose7.537with NAD
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
0.037 μmol/min/mg7.537toward GDP-4-keto-3,6-dideoxy-D-mannose, with NADP
0.015 μmol/min/mg7.537toward GDP-4-keto-3,6-dideoxy-D-mannose, with NAD

Pathway

Nucleotide-sugar metabolism; GDP-L-colitose biosynthesis.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site7-13NADP+ (UniProtKB | ChEBI)
Binding site101-104NADP+ (UniProtKB | ChEBI)
Site103Important for catalytic activity
Site105Important for catalytic activity
Active site132Proton donor/acceptor
Binding site136NADP+ (UniProtKB | ChEBI)
Binding site160-163NADP+ (UniProtKB | ChEBI)
Binding site176NADP+ (UniProtKB | ChEBI)
Binding site184substrate
Binding site199substrate
Binding site206substrate

GO annotations

AspectTerm
Molecular FunctionGDP-L-fucose synthase activity
Molecular Functionisomerase activity
Molecular FunctionNADP+ binding
Molecular Functionoxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Biological Process'de novo' GDP-L-fucose biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    GDP-L-colitose synthase
  • EC number

Gene names

    • Name
      colC
    • Synonyms
      colB, fcl

Organism names

  • Taxonomic identifier
  • Strain
    • H720/86
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Yersiniaceae > Yersinia

Accessions

  • Primary accession
    G4WJD3

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004356891-308GDP-L-colitose synthase

Interaction

Subunit

Homodimer.

Structure

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    308
  • Mass (Da)
    34,768
  • Last updated
    2011-12-14 v1
  • Checksum
    E4B53BB5F487D54E
MKILLTGAGGMVGKNILAHTKSKDYEFITPSSKELDLLEKKHITTYLKHHKPNFIIHAAGIVGGIHANINNPVKFLVENMQMGINLLTAAKDNNIRKLLNLGSSCMYPKDCDSGLTEDMILTGELESTNEGYALAKITSAKLCEYINREDSEFQYKTAIPCNLYGKYDKFDENNSHMIPAVIKKIVTAIETGKSEVEIWGDGEARREFMYAEDLADFIFYTINNFTKMPQNINVGLGQDYTITEYYKVIAKILGYKGTFVYDKSKPVGMRRKLIDNTLLSEFGWSNKVDLESGISKTCQYFLNEKNND

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HQ456392
EMBL· GenBank· DDBJ
AEP25495.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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