G3KLH4 · ADAC_ASPNG

  • Protein
    FAD-dependent monooxygenase adaC
  • Gene
    adaC
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    3/5

Function

function

FAD-dependent monooxygenase; part of the gene cluster that mediates the biosynthesis of the linear tetracyclic TAN-1612 neuropeptide Y receptor antagonist (PubMed:21866960).
The decaketide backbone of TAN-1612 is synthesized by the non-reducing polyketide synthase adaA via condensation of one acetyl-CoA starter unit with 9 malonyl-CoA units. The FAD-dependent monooxygenase adaC then performs hydroxylation at C2 while the polaketide chain is still attached to the NRPKS adaA (PubMed:21866960).
The alpha-hydroxylation step at C2 appears to be crucial for the following C18-C1 Claisen cyclization and release of the C9-hydroxyl version of TAN-1612 from the NRPKS adaA, two steps performed by the lactamase-like protein adaB (PubMed:21866960).
Finally, the O-methyltransferase adaD performs the C9 O-methylation to complete the biosynthesis of TAN-1612 (PubMed:21866960).

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site32FAD (UniProtKB | ChEBI)
Binding site43FAD (UniProtKB | ChEBI)
Binding site115FAD (UniProtKB | ChEBI)
Binding site325FAD (UniProtKB | ChEBI)
Binding site338FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionFAD binding
Molecular Functionmonooxygenase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    FAD-dependent monooxygenase adaC
  • EC number
  • Alternative names
    • 2-acetyl-2-decarboxamidoanthrotainin biosynthesis cluster protein C

Gene names

    • Name
      adaC
    • ORF names
      ATCC64974_92720

Organism names

  • Taxonomic identifier
  • Strains
    • ATCC 1015 / NV DSM 2061
    • ATCC 64974 / FGSC A733 / N402
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Circumdati

Accessions

  • Primary accession
    G3KLH4

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004463471-414FAD-dependent monooxygenase adaC

Proteomic databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    414
  • Mass (Da)
    45,561
  • Last updated
    2011-11-16 v1
  • Checksum
    18980F105D1DC4E3
MTPPILIIGAGLSGLTISRILTNASIPNIVFEASTPDRSQGYAISLREWGYTSLLTALGDLPLRSLTRGVAPDRILGGTGWIDQALRDNHTGNLLVAPDPEAKQCIVRANRNALRTWIADSGDEEVDIRYGHRLRSVQGSMGNVTATFDNGAKYQGSLVIAADGVHSSVRSQILPHVSPDIVPVVVYHGELELPRKEFDNLIRPHSGPSNILAGVGDGFNTPITVCNITPTHVHLDWSYSRPSTENKENKDPLYRPHVSAAEAKQIPPALLEEIASRDLARPWSQLLNAEALPTHRVFNWVSRCVSVTREDVNAAQKQGVVFIGDSWHAMPIFGGEGGNHALVDAVELAEALTGKEGNLDAAVTGYYDRAWRRCQEAVRRSRQRFFQLHRPMREWMEIAEKKKMMAAMKGVEAH

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JN257714
EMBL· GenBank· DDBJ
AEN83887.1
EMBL· GenBank· DDBJ
Genomic DNA
OGUI01000016
EMBL· GenBank· DDBJ
SPB51662.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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