G2Q9A6 · MYCB_THET4

Function

function

Diels-Alderase; part of the gene cluster that mediates the biosynthesis of myceliothermophins, mycotoxins that contain a trans-fused decalin ring system connected to a conjugated 3-pyrrolin-2-one moiety and that have potential anti-tumor properties (PubMed:27960349).
The polyketide synthase module (PKS) of the PKS-NRPS mycA is responsible for the synthesis of the octaketide backbone (PubMed:27960349).
The downstream nonribosomal peptide synthetase (NRPS) module then amidates the carboxyl end of the octaketide with a leucine (PubMed:27960349).
A reductase-like domain (R) at the C-terminus catalyzes the reductive release of the polyketide-amino acid intermediate (PubMed:27960349).
Because mycA lacks a designated enoylreductase (ER) domain, the required activity is provided the enoyl reductase mycC (PubMed:27960349).
Following mycA-catalyzed construction and release of aminoacyl polyketide aldehyde, Knoevenagel condensation yields the expected ketone (PubMed:27960349).
This C18 keto acyclic precursor is the substrate of the Diels-Alderase mycB, that catalyzes the Diels-Alder cycloaddition to produce myceliothermophin E (PubMed:27960349).
A yet unknown oxygenase involved in the production of myceliothermophin A, via substitution with a hydroxyl group at the C21, has still to be identified (PubMed:27960349).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
75 μMthe C18 keto acyclic precursor

Pathway

Mycotoxin biosynthesis.

GO annotations

AspectTerm
Molecular Functionisomerase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Diels-Alderase mycB
  • EC number
  • Alternative names
    • Myceliothermophin biosynthesis cluster protein B

Gene names

    • Name
      mycB
    • ORF names
      MYCTH_114667

Organism names

Accessions

  • Primary accession
    G2Q9A6

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Completely abolishes the production of myceliothermophin E, but leads to the accumulation of its C18 keto acyclic precursor.

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-18
ChainPRO_500343605819-440Diels-Alderase mycB
Glycosylation80N-linked (GlcNAc...) asparagine
Glycosylation155N-linked (GlcNAc...) asparagine
Glycosylation332N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Structure

Family & Domains

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    440
  • Mass (Da)
    49,347
  • Last updated
    2011-11-16 v1
  • Checksum
    5DC67D6456AD0FB3
MGYLVKLACGLLLPLATADVNLAQQPFGEFEWQPKFDTPSGDHTSCAVSRLSAYEVGEGPVSFSLSPTEHPEIPKLSTINSTVWEQWEFDAVSESGTGSVLMGFSRDPSYSFFGQGNLRVEFYMTLEDGTRIQELEYTESSTVIDCPDSIRGIWNSSDRSYAFEISRDMQRARVWWDTGREKGSIAIESTTTPHLADGEIWPPEEGESRVKKSWPSVKLSPGLYMSQPIAGGKVTAHVQIGKKTMSITGYGAHSRLWAENSWFKICRGWQIMRGFLGPYTISYWRPLSRLDDWVPYFAAHLFKHGRLVFTSQVGAPSQQVDYTQFHSDFSGNVSGSLADKATGRVLEFVSPSTGKTWRFHHRHFAKMFEMGFGGGRGLTGFLDEIVGGEVGTDEEFTGRGFSEQVLLPDEIKQWQIWVVYGIGFLGRWKNTVTNLVLSIW

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP003003
EMBL· GenBank· DDBJ
AEO57198.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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