G2J2L6 · G2J2L6_PSEUL
- Protein2-methylisocitrate lyase
- GeneprpB
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids291 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate.
Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate via an alpha-carboxy-carbanion intermediate.
Catalytic activity
- (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
Cofactor
Pathway
Organic acid metabolism; propanoate degradation.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 43-45 | substrate | ||||
Sequence: SGG | ||||||
Binding site | 83 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 85 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 120-121 | substrate | ||||
Sequence: CG | ||||||
Binding site | 155 | substrate | ||||
Sequence: R | ||||||
Binding site | 185 | substrate | ||||
Sequence: E | ||||||
Binding site | 207-209 | substrate | ||||
Sequence: NIT | ||||||
Binding site | 238 | substrate | ||||
Sequence: R | ||||||
Binding site | 267 | substrate | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | magnesium ion binding | |
Molecular Function | methylisocitrate lyase activity | |
Biological Process | propionate catabolic process, 2-methylcitrate cycle |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-methylisocitrate lyase
- EC number
- Short names2-MIC ; MICL
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Neisseriales > Chromobacteriaceae > Pseudogulbenkiania
Accessions
- Primary accessionG2J2L6
Proteomes
Interaction
Structure
Sequence
- Sequence statusComplete
- Length291
- Mass (Da)31,673
- Last updated2011-11-16 v1
- Checksum0BBD80EAD27DB822
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP012224 EMBL· GenBank· DDBJ | BAK76392.1 EMBL· GenBank· DDBJ | Genomic DNA |