G2J2L6 · G2J2L6_PSEUL

Function

function

Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate.
Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate via an alpha-carboxy-carbanion intermediate.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Organic acid metabolism; propanoate degradation.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site43-45substrate
Binding site83Mg2+ (UniProtKB | ChEBI)
Binding site85Mg2+ (UniProtKB | ChEBI)
Binding site120-121substrate
Binding site155substrate
Binding site185substrate
Binding site207-209substrate
Binding site238substrate
Binding site267substrate

GO annotations

AspectTerm
Molecular Functionmagnesium ion binding
Molecular Functionmethylisocitrate lyase activity
Biological Processpropionate catabolic process, 2-methylcitrate cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    2-methylisocitrate lyase
  • EC number
  • Short names
    2-MIC
    ; MICL
  • Alternative names
    • (2R,3S)-2-methylisocitrate lyase

Gene names

    • Name
      prpB
    • Ordered locus names
      NH8B_1570

Organism names

Accessions

  • Primary accession
    G2J2L6

Proteomes

Interaction

Subunit

Homotetramer; dimer of dimers.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    291
  • Mass (Da)
    31,673
  • Last updated
    2011-11-16 v1
  • Checksum
    0BBD80EAD27DB822
MHTPGNRFRQAVEQEKPLQVMGAVNAYAARLAEHAGFKALYLSGGGVAACSCGIPDLGITTLEDVLIDVRRITEATELPLLVDIDTGWGGAFNIARAIRSLEKAGAAAVHIEDQVQQKRCGHRPNKAIVSKDEMVDRIKAAVDARRDDSFVIMARTDALAVEGLDAAIERAVACVEAGADMIFPEAITDLAMYKRFAEAVKVPVLANITEFGSTPLYTTQELGANGVSLVLYPLSAFRAMSKAALEVYSAIRQDGTQKNVIDKMQTRMELYDHLGYHAYEQKLDALFSQEK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012224
EMBL· GenBank· DDBJ
BAK76392.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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