E3WPP8 · TRM5_ANODA
- ProteintRNA (guanine(37)-N1)-methyltransferase
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids500 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Catalytic activity
- guanosine37 in tRNA + S-adenosyl-L-methionine = H+ + N1-methylguanosine37 in tRNA + S-adenosyl-L-homocysteine
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 215 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 253-254 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DL | ||||||
Binding site | 281-282 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DA | ||||||
Binding site | 312 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial matrix | |
Cellular Component | nucleus | |
Molecular Function | tRNA (guanine(37)-N1)-methyltransferase activity | |
Biological Process | mitochondrial tRNA methylation | |
Biological Process | tRNA N1-guanine methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (guanine(37)-N1)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Nematocera > Culicoidea > Culicidae > Anophelinae > Anopheles
Accessions
- Primary accessionE3WPP8
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Predominantly in the mitochondria and in the nucleus.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000414127 | 1-500 | tRNA (guanine37-N1)-methyltransferase | |||
Sequence: MCADLLPPATVRGMEQLDRDAFAKTVRVPHLIIPEATNLNSAARALKQYLLKMEHYKPIRSEERKITLHPIPVKQWEDLPVEPLKELGIEKDCLVWEEIKLSYENYKYDLILKAVLPENQEGLSAFSKIGHIIHLNLKNHLMPYRRLIGEVLMDKVADCRTVVNKSNSIQNTYRNFEMELICGVPEYEVSIKENGCTYKFNFSRVYWNPRLSTEHQKITDMLEEGDLLYDLYAGVGPFTVPAAKRGCTVIANDLNPDSYSALVINCGLNKVMRNVKCYNMDAVDFIKVELRNDLLAKLADDKFQGNIHITMNLPAMAVEHLVHFPGLFSGESIELRIKPLVHVYCFAQGADDKKPIAQQKVEQWLGVEVTDMLKEITFVRNVAPNKDMMRVRNPSALGMAKKANKLKDQPKQLAKKAKNVFAVSQTKKGNLKKTKEVAAKLKKINVQDKREKVDANFKTLHAQIVAKKTPKPAPRPLPAKNKTTPDTNKMETDLTKLEMK |
Interaction
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 463-500 | Disordered | ||||
Sequence: QIVAKKTPKPAPRPLPAKNKTTPDTNKMETDLTKLEMK | ||||||
Compositional bias | 486-500 | Basic and acidic residues | ||||
Sequence: DTNKMETDLTKLEMK |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length500
- Mass (Da)56,886
- Last updated2011-02-08 v1
- ChecksumA1144DE52502CB31
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 486-500 | Basic and acidic residues | ||||
Sequence: DTNKMETDLTKLEMK |
Keywords
- Technical term