E1W9M1 · END8_SALTS

Function

function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as a DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site2Schiff-base intermediate with DNA
Active site3Proton donor
Active site53Proton donor; for beta-elimination activity
Binding site70DNA (UniProtKB | ChEBI)
Binding site125DNA (UniProtKB | ChEBI)
Binding site169DNA (UniProtKB | ChEBI)
Active site253Proton donor; for delta-elimination activity

GO annotations

AspectTerm
Molecular Functionclass I DNA-(apurinic or apyrimidinic site) endonuclease activity
Molecular Functiondamaged DNA binding
Molecular Functionoxidized pyrimidine nucleobase lesion DNA N-glycosylase activity
Molecular Functionzinc ion binding
Biological Processbase-excision repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Endonuclease 8
  • Alternative names
    • DNA glycosylase/AP lyase Nei
      (EC:3.2.2.-
      , EC:4.2.99.18
      ) . EC:3.2.2.- (UniProtKB | ENZYME | Rhea)
      , EC:4.2.99.18 (UniProtKB | ENZYME | Rhea)
    • DNA-(apurinic or apyrimidinic site) lyase Nei
    • Endonuclease VIII

Gene names

    • Name
      nei
    • Ordered locus names
      SL1344_0710

Organism names

Accessions

  • Primary accession
    E1W9M1
  • Secondary accessions
    • Q8ZQU6
    • Q9F612

Proteomes

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00004054162-263Endonuclease 8

Structure

Family & Domains

Features

Showing features for zinc finger.

TypeIDPosition(s)Description
Zinc finger229-263FPG-type

Sequence similarities

Belongs to the FPG family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    263
  • Mass (Da)
    29,863
  • Last updated
    2010-11-30 v1
  • Checksum
    0BA96E876F2F19FE
MPEGPEIRRAADNLEAAIKGKPLTDVWFAFAQLKPYESQLTGQLVTRIETRGKALLTHFSNGLTLYSHNQLYGVWRVIDTGEIPQTTRILRVRLQTADKTILLYSASDIEMLTAEQLTTHPFLQRVGPDVLDARLTPEEVKARLLSPRFRNRQFSGLLLDQSFLAGLGNYLRVEILWQVGLTGQHKAKDLNEAQLNALSHALLDIPRLSYTTRGQADENKHHGALFRFKLFHRDGEACERCGGIIEKTTLSSRPFYWCPHCQK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FQ312003
EMBL· GenBank· DDBJ
CBW16808.1
EMBL· GenBank· DDBJ
Genomic DNA
AF300989
EMBL· GenBank· DDBJ
AAG28771.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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