D7Y2H3 · CAP7_ECOM1
- ProteinCD-NTase-associated protein 7
- Genecap7
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids172 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Sensor protein of a CBASS antivirus system (PubMed:31932165).
CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type III-C(AAA) CBASS system (PubMed:32839535).
CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type III-C(AAA) CBASS system (PubMed:32839535).
Binds to a closure peptide (consensus His-Xaa-Xaa-Ile-Leu-Leu-Thr), which allows it to activate CdnC for second messenger synthesis (PubMed:31932165).
Protects E.coli strain JP313 against bacteriophage lambda cI- infection. When the cdnC-cap7-cap6-nucC operon is transformed into a susceptible strain it confers bacteriophage immunity. Mutations in the sensor (Cap7 also called HORMA) or effector proteins (CdnC, NucC) but not the disassembly protein (Cap6 also called Trip13) no longer confer immunity. The presence of the intact operon leads to culture collapse and cell death, which occurs before the phage has finished its replication cycle, thus protecting non-infected bacteria by aborting the phage infection and preventing its propagation.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Biological Process | defense response to virus |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameCD-NTase-associated protein 7
- Short namesCap7
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionD7Y2H3
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 1-12 | Fully active in second messenger formation. Cap7:CdnC complex is no longer disassembled by Cap6. | ||||
Sequence: Missing | ||||||
Mutagenesis | 32 | Binds to CdnC. | ||||
Sequence: R → A | ||||||
Mutagenesis | 32-35 | No longer binds to CdnC. No longer confers phage immunity. | ||||
Sequence: RMQR → AMQA | ||||||
Mutagenesis | 35 | Binds to CdnC. | ||||
Sequence: R → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000451840 | 1-172 | CD-NTase-associated protein 7 | |||
Sequence: MSSYSYTVAETQTFSVTHARHMAAKVATDLRRMQRFYGYPSDADIEAYEEELVVFLKAGYLGEVSYGFQKNNNWIEPTLRYTAGDLLGSGTDDDPGKIRPGKDVSGASFYSFMTYSSKYLNATQSEKDTALKDLPFKRVGAQSPGINGYLENDKTYSAGGRSLTRTSVRNFV |
Interaction
Subunit
Forms complexes with CdnC with 1:1 and 2:2 stoichimetry, and a 1:1:6 CdnC:Cap7:Cap6 complex.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 141-172 | Required for binding to CdnC and to confer phage immunity | ||||
Sequence: AQSPGINGYLENDKTYSAGGRSLTRTSVRNFV |
Domain
In the disassembly complex (PDB:6P8V) Cap6 (also called Trip13) crystallizes as a right-handed spiral; the top 4 subunits bind ATP while the bottom 2 do not. A CdnC monomer lies along the surface of the hexamer at the interface of subunits 5 and 6, with Cap7 (this protein) at its tip, over the central hexamer pore. The N-terminus of Cap7 extends into the pore, contacting 5/6 Cap6 subunits.
Sequence similarities
Belongs to the bacterial HORMA family. HORMA1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length172
- Mass (Da)19,144
- Last updated2021-02-10 v2
- Checksum1B7E2104ACDB2D44
Sequence caution
Keywords
- Technical term