D7Y2H3 · CAP7_ECOM1

Function

function

Sensor protein of a CBASS antivirus system (PubMed:31932165).
CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophage. The CD-NTase protein synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type III-C(AAA) CBASS system (PubMed:32839535).
Binds to a closure peptide (consensus His-Xaa-Xaa-Ile-Leu-Leu-Thr), which allows it to activate CdnC for second messenger synthesis (PubMed:31932165).
Protects E.coli strain JP313 against bacteriophage lambda cI- infection. When the cdnC-cap7-cap6-nucC operon is transformed into a susceptible strain it confers bacteriophage immunity. Mutations in the sensor (Cap7 also called HORMA) or effector proteins (CdnC, NucC) but not the disassembly protein (Cap6 also called Trip13) no longer confer immunity. The presence of the intact operon leads to culture collapse and cell death, which occurs before the phage has finished its replication cycle, thus protecting non-infected bacteria by aborting the phage infection and preventing its propagation.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Biological Processdefense response to virus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    CD-NTase-associated protein 7
  • Short names
    Cap7
  • Alternative names
    • Bacterial HORMA sensor protein

Gene names

    • Name
      cap7
    • Synonyms
      HORMA1
    • ORF names
      HMPREF9540_01759

Organism names

Accessions

  • Primary accession
    D7Y2H3

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis1-12Fully active in second messenger formation. Cap7:CdnC complex is no longer disassembled by Cap6.
Mutagenesis32Binds to CdnC.
Mutagenesis32-35No longer binds to CdnC. No longer confers phage immunity.
Mutagenesis35Binds to CdnC.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004518401-172CD-NTase-associated protein 7

Interaction

Subunit

Forms complexes with CdnC with 1:1 and 2:2 stoichimetry, and a 1:1:6 CdnC:Cap7:Cap6 complex.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region141-172Required for binding to CdnC and to confer phage immunity

Domain

In the disassembly complex (PDB:6P8V) Cap6 (also called Trip13) crystallizes as a right-handed spiral; the top 4 subunits bind ATP while the bottom 2 do not. A CdnC monomer lies along the surface of the hexamer at the interface of subunits 5 and 6, with Cap7 (this protein) at its tip, over the central hexamer pore. The N-terminus of Cap7 extends into the pore, contacting 5/6 Cap6 subunits.

Sequence similarities

Belongs to the bacterial HORMA family. HORMA1 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    172
  • Mass (Da)
    19,144
  • Last updated
    2021-02-10 v2
  • Checksum
    1B7E2104ACDB2D44
MSSYSYTVAETQTFSVTHARHMAAKVATDLRRMQRFYGYPSDADIEAYEEELVVFLKAGYLGEVSYGFQKNNNWIEPTLRYTAGDLLGSGTDDDPGKIRPGKDVSGASFYSFMTYSSKYLNATQSEKDTALKDLPFKRVGAQSPGINGYLENDKTYSAGGRSLTRTSVRNFV

Sequence caution

The sequence EFJ98157.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ADTL01000141
EMBL· GenBank· DDBJ
EFJ98157.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Similar Proteins

Disclaimer

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