D4AN96 · SWNR_ARTBC

Function

function

Oxidoreductase; part of the gene cluster that mediates the biosynthesis of swainsonine (SW), a cytotoxic fungal alkaloid and a potential cancer therapy drug (PubMed:28381497).
Swainsonine production occurs via a multibranched pathway and is dispensable for fungal colonization of plants and infection of insect hosts (By similarity).
The first step of swainsonine biosynthesis is the production of the precursor pipecolic acid (PA) via conversion of L-lysine (Lys) to 1-piperideine-6-carboxylate (P6C) by the aminotransferase swnA, the latter being further reduced to PA by the reductase swnR (By similarity).
The PKS-NRPS hybrid synthetase swnK uptakes and condensates PA and malonyl-CoA with and without skipping of the ketoreductase (KR) domain in order to produce 3 intermediates, 1-oxoindolizidine, (1S)-1-hydroxyindolizin, and (1R)-1-hydroxyindolizine; with the transisomer (1S)-1-hydroxyindolizin being predominant (By similarity).
The terminal thioester reductase (TE) domain of swnK is involved in reduction of the thioester bond to release the intermediate aldehydes (By similarity).
The oxidoreductase swnN could contribute to the reduction of 1-oxoindolizidine to (1S)-1-hydroxyindolizin and (1R)-1-hydroxyindolizine, contributing to the major route of SW production (By similarity).
The dioxygenase swnH2 would be responsible for the oxidization of (1R)-1-hydroxyindolizine into (1R,2S)-1,2-dihydroxyindolizine and of (1S)-1-hydroxyindolizin to yield both (1R,2S)-1,2-dihydroxyindolizine and (1S,2S)-1,2-dihydroxyindolizine (By similarity).
The dioxygenase swnH1 then performs the conversion of the 1,2-dihydroxyindolizine epimers to SW (By similarity).

Catalytic activity

Pathway

Mycotoxin biosynthesis.

GO annotations

AspectTerm
Molecular FunctionL-pipecolate oxidase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Oxidoreductase swnR
  • EC number
  • Alternative names
    • Swainsonine biosynthesis gene cluster protein R

Gene names

    • Name
      swnR
    • ORF names
      ARB_05701

Organism names

Accessions

  • Primary accession
    D4AN96

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004411871-305Oxidoreductase swnR

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    305
  • Mass (Da)
    34,471
  • Last updated
    2010-05-18 v1
  • Checksum
    BB673F39871C6C8F
MKVAIAGYGDLTRYICEEFVQAGHELVILTRSFKPQIESPGISQAITDYTISSLKATLADCEVLISTIGDMSNAYTSVHHTLIQACQESPKCKRFIPAEFAVNIETYPDEPGFYYAIHEPVRETLRNQTNLEWTLVCIGWLADYFVPSKNRYIKDIDECHPINWKANKAVIPGTGNEPVDFTWARDVAKGLASLIQAPTGSWEPYTFMSGERSCWNDMAALIKEKYRPDMPIEHVSLHATANMIKAAKDEDTLILADYYLLSISQACAIPQDKAKAHKEKFFPHIHFRTLREGLSQFDEKPNSIM

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
ABSU01000003
EMBL· GenBank· DDBJ
EFE35657.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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