D3QW22 · TIR_ECOCB
- ProteinTranslocated intimin receptor Tir
- Genetir
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids556 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Multifunctional protein that is required for efficient pedestal formation in host epithelial cells during infection. The extracellular region acts as a receptor for bacterial intimin, allowing the bacterium to attach tightly to the host-cell surface. Simultaneously, the intracellular region initiates a signaling cascade in the host cell, which leads to actin polymerization and formation of actin pedestals at the sites of bacterial adhesion (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | host cell plasma membrane | |
Cellular Component | membrane |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameTranslocated intimin receptor Tir
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionD3QW22
Subcellular Location
UniProt Annotation
GO Annotation
Host cell membrane ; Multi-pass membrane protein
Note: Secreted via the type III secretion system (T3SS). Released into the host cytoplasm via T3SS and then independently inserts into the plasma membrane from a cytoplasmic location. In host cells, localizes to the tip of the actin pedestal (By similarity).
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-230 | Cytoplasmic | ||||
Sequence: MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGSRALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQIGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGGAGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQTSTSTSSLRSDPK | ||||||
Transmembrane | 231-251 | Helical | ||||
Sequence: LWLALGTVATGLIGLAATGIV | ||||||
Topological domain | 252-362 | Extracellular | ||||
Sequence: QALALTPEPDSPTTTDPDAAASATETATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAIENNAQAQKKYDEQQAKRQEELKVSSGAG | ||||||
Transmembrane | 363-383 | Helical | ||||
Sequence: YGLSGALILGGGIGVAVTAAL | ||||||
Topological domain | 384-556 | Cytoplasmic | ||||
Sequence: HRKNQPVEQTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLLGNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000414058 | 1-556 | Translocated intimin receptor Tir | |||
Sequence: MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGSRALFTPVRNSMADSGDNRASDVPGLPVNPMRLAASEITLNDGFEVLHDHGPLDTLNRQIGSSVFRVETQEDGKHIAVGQRNGVETSVVLSDQEYARLQSIDPEGKDKFVFTGGRGGAGHAMVTVASDITEARQRILELLEPKGTGESKGAGESKGVGELRESNSGAENTTETQTSTSTSSLRSDPKLWLALGTVATGLIGLAATGIVQALALTPEPDSPTTTDPDAAASATETATRDQLTKEAFQNPDNQKVNIDELGNAIPSGVLKDDVVANIEEQAKAAGEEAKQQAIENNAQAQKKYDEQQAKRQEELKVSSGAGYGLSGALILGGGIGVAVTAALHRKNQPVEQTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSSIGTVQNPYADVKTSLHDSQVPTSNSNTSVQNMGNTDSVVYSTIQHPPRDTTDNGARLLGNPSAGIQSTYARLALSGGLRHDMGGLTGGSNSAVNTSNNPPAPGSHRFV |
Post-translational modification
Phosphorylated by host kinases.
Keywords
- PTM
Interaction
Subunit
Interacts with intimin and host proteins.
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-44 | Disordered | ||||
Sequence: MPIGNLGHNPNVNNSIPPAPPLPSQTDGAGGRGQLINSTGPLGS | ||||||
Compositional bias | 30-44 | Polar residues | ||||
Sequence: GGRGQLINSTGPLGS | ||||||
Region | 182-226 | Disordered | ||||
Sequence: LLEPKGTGESKGAGESKGVGELRESNSGAENTTETQTSTSTSSLR | ||||||
Compositional bias | 206-226 | Polar residues | ||||
Sequence: SNSGAENTTETQTSTSTSSLR | ||||||
Region | 257-280 | Disordered | ||||
Sequence: TPEPDSPTTTDPDAAASATETATR | ||||||
Compositional bias | 258-280 | Polar residues | ||||
Sequence: PEPDSPTTTDPDAAASATETATR | ||||||
Region | 388-449 | Disordered | ||||
Sequence: QPVEQTTTTTTTTTSARTVENKPANNTPAQGNVDTPGSEDTMESRRSSMASTSSTFFDTSSI | ||||||
Motif | 454-456 | Essential for actin pedestal formation | ||||
Sequence: NPY | ||||||
Region | 531-556 | Disordered | ||||
Sequence: GGLTGGSNSAVNTSNNPPAPGSHRFV | ||||||
Compositional bias | 535-550 | Polar residues | ||||
Sequence: GGSNSAVNTSNNPPAP |
Sequence similarities
Belongs to the Tir receptor family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length556
- Mass (Da)57,820
- Last updated2010-04-20 v1
- Checksum4A6446923D83A03C
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 30-44 | Polar residues | ||||
Sequence: GGRGQLINSTGPLGS | ||||||
Compositional bias | 206-226 | Polar residues | ||||
Sequence: SNSGAENTTETQTSTSTSSLR | ||||||
Compositional bias | 258-280 | Polar residues | ||||
Sequence: PEPDSPTTTDPDAAASATETATR | ||||||
Compositional bias | 535-550 | Polar residues | ||||
Sequence: GGSNSAVNTSNNPPAP |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001846 EMBL· GenBank· DDBJ | ADD58883.1 EMBL· GenBank· DDBJ | Genomic DNA |