C9RFN5 · C9RFN5_METVM

Function

function

Catalyzes the insertion of Co2+ into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni2+ into sirohydrochlorin to yield Ni-sirohydrochlorin.

Catalytic activity

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site9Proton acceptor
Binding site9Co2+ (UniProtKB | ChEBI)
Binding site9Ni2+ (UniProtKB | ChEBI)
Binding site45substrate
Binding site70-75substrate
Binding site75Co2+ (UniProtKB | ChEBI)
Binding site75Ni2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functioncobalt ion binding
Molecular Functionnickel cation binding
Molecular Functionsirohydrochlorin cobaltochelatase activity
Biological Processanaerobic cobalamin biosynthetic process
Biological Processmethanogenesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Sirohydrochlorin cobaltochelatase
  • EC number
  • Alternative names
    • CbiXS
    • Sirohydrochlorin nickelchelatase
      (EC:4.99.1.11
      ) . EC:4.99.1.11 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      cbiX
    • Synonyms
      cfbA
    • Ordered locus names
      Metvu_0528

Organism names

Accessions

  • Primary accession
    C9RFN5

Proteomes

Interaction

Subunit

Homotetramer; dimer of dimers.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the CbiX family. CbiXS subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    141
  • Mass (Da)
    16,194
  • Last updated
    2009-11-24 v1
  • Checksum
    9B9A9CF7E6A1F30D
MEALVLVGHGSRLPYSKELLVNLAEKIKEKNLFPIVEIGMMEFNEPTIPEAVKKAIDQGAKKIIVVPVFLAHGIHTTRDIPKMLGLIEDTHEDDHHHHHHHHHHHDFEKLEIPEDVEIVYREPIGADDRIVDIIIDRAFGR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001787
EMBL· GenBank· DDBJ
ACX72387.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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