C9RFN5 · C9RFN5_METVM
- ProteinSirohydrochlorin cobaltochelatase
- GenecbiX
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids141 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the insertion of Co2+ into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni2+ into sirohydrochlorin to yield Ni-sirohydrochlorin.
Catalytic activity
- Ni-sirohydrochlorin + 2 H+ = sirohydrochlorin + Ni2+
Pathway
Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 9 | Proton acceptor | ||||
Sequence: H | ||||||
Binding site | 9 | Co2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 9 | Ni2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 45 | substrate | ||||
Sequence: E | ||||||
Binding site | 70-75 | substrate | ||||
Sequence: LAHGIH | ||||||
Binding site | 75 | Co2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 75 | Ni2+ (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | cobalt ion binding | |
Molecular Function | nickel cation binding | |
Molecular Function | sirohydrochlorin cobaltochelatase activity | |
Biological Process | anaerobic cobalamin biosynthetic process | |
Biological Process | methanogenesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSirohydrochlorin cobaltochelatase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Methanomada group > Methanococci > Methanococcales > Methanocaldococcaceae > Methanocaldococcus
Accessions
- Primary accessionC9RFN5
Proteomes
Interaction
Structure
Sequence
- Sequence statusComplete
- Length141
- Mass (Da)16,194
- Last updated2009-11-24 v1
- Checksum9B9A9CF7E6A1F30D
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001787 EMBL· GenBank· DDBJ | ACX72387.1 EMBL· GenBank· DDBJ | Genomic DNA |