C7SDN9 · N7OMT_PAPSO
- ProteinNorreticuline-7-O-methyltransferase
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids357 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Involved in the biosynthesis of benzylisoquinoline alkaloids (PubMed:19500305).
Catalyzes specifically the methylation of norreticuline at position seven to produce norlaudanine (PubMed:19500305).
No activity with norcoclaurine, reticuline, norlaudanosoline, norisoorientaline, scoulerine, salutaridinol, oripavine, salsolinol, codeine or morphine (PubMed:19500305).
Involved in papaverine biosynthesis (PubMed:22725256, PubMed:23738019).
Catalyzes specifically the methylation of norreticuline at position seven to produce norlaudanine (PubMed:19500305).
No activity with norcoclaurine, reticuline, norlaudanosoline, norisoorientaline, scoulerine, salutaridinol, oripavine, salsolinol, codeine or morphine (PubMed:19500305).
Involved in papaverine biosynthesis (PubMed:22725256, PubMed:23738019).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
44 μM | norreticuline | |||||
19 μM | S-adenosyl-L-methionine |
kcat is 0.074 sec-1 for norreticuline.
pH Dependence
Optimum pH is between 7.0 and 9.5.
Temperature Dependence
Optimum temperature is 35 degrees Celsius.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | O-methyltransferase activity | |
Molecular Function | protein dimerization activity | |
Molecular Function | S-adenosylmethionine-dependent methyltransferase activity | |
Biological Process | alkaloid metabolic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNorreticuline-7-O-methyltransferase
- EC number
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Ranunculales > Papaveraceae > Papaveroideae > Papaver
Accessions
- Primary accessionC7SDN9
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000433986 | 1-357 | Norreticuline-7-O-methyltransferase | |||
Sequence: MEVVSQIDQENQAIIWKQIYGFSESLLLKCAVQCEIAETIHNHGTPMSILELAAKLPIDQPVNIDRLYRVMRYLVHQKLFNKEVISTLNGGTVQVTEKYWLAPPAKYLIRGSQQSMVPSVLGIIDEDMFAPWHILKDSLTGECNIFETALGKSISVYMSENPEMNQISNGAMAFDSGLVTSHLVNECKSVFGDEIKTLVDVGGGTGTALRAISKAFPNIKCTLFDLPHVIADSPEIPTITKVSGDMFKSIPSADAIFMKNILHDWNDDECIQILKRCKDVVSAGGKLIMVEMVLDEDSFHPYSKLRLTSDIDMMVNNGGKERTEKEWEKLFDAAGFASCKFTQMSVGFAAQSIIEVY |
Expression
Tissue specificity
Expressed instems, leaves, roots and seedlings.
Structure
Sequence
- Sequence statusComplete
- Length357
- Mass (Da)39,701
- Last updated2009-10-13 v1
- ChecksumD1682F6B4E08953F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FJ156103 EMBL· GenBank· DDBJ | ACN88562.1 EMBL· GenBank· DDBJ | Genomic DNA |