C7SDN9 · N7OMT_PAPSO

Function

function

Involved in the biosynthesis of benzylisoquinoline alkaloids (PubMed:19500305).
Catalyzes specifically the methylation of norreticuline at position seven to produce norlaudanine (PubMed:19500305).
No activity with norcoclaurine, reticuline, norlaudanosoline, norisoorientaline, scoulerine, salutaridinol, oripavine, salsolinol, codeine or morphine (PubMed:19500305).
Involved in papaverine biosynthesis (PubMed:22725256, PubMed:23738019).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
44 μMnorreticuline
19 μMS-adenosyl-L-methionine
kcat is 0.074 sec-1 for norreticuline.

pH Dependence

Optimum pH is between 7.0 and 9.5.

Temperature Dependence

Optimum temperature is 35 degrees Celsius.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site225S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site263Proton acceptor

GO annotations

AspectTerm
Molecular FunctionO-methyltransferase activity
Molecular Functionprotein dimerization activity
Molecular FunctionS-adenosylmethionine-dependent methyltransferase activity
Biological Processalkaloid metabolic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Norreticuline-7-O-methyltransferase
  • EC number

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Ranunculales > Papaveraceae > Papaveroideae > Papaver

Accessions

  • Primary accession
    C7SDN9

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004339861-357Norreticuline-7-O-methyltransferase

Expression

Tissue specificity

Expressed instems, leaves, roots and seedlings.

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    357
  • Mass (Da)
    39,701
  • Last updated
    2009-10-13 v1
  • Checksum
    D1682F6B4E08953F
MEVVSQIDQENQAIIWKQIYGFSESLLLKCAVQCEIAETIHNHGTPMSILELAAKLPIDQPVNIDRLYRVMRYLVHQKLFNKEVISTLNGGTVQVTEKYWLAPPAKYLIRGSQQSMVPSVLGIIDEDMFAPWHILKDSLTGECNIFETALGKSISVYMSENPEMNQISNGAMAFDSGLVTSHLVNECKSVFGDEIKTLVDVGGGTGTALRAISKAFPNIKCTLFDLPHVIADSPEIPTITKVSGDMFKSIPSADAIFMKNILHDWNDDECIQILKRCKDVVSAGGKLIMVEMVLDEDSFHPYSKLRLTSDIDMMVNNGGKERTEKEWEKLFDAAGFASCKFTQMSVGFAAQSIIEVY

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FJ156103
EMBL· GenBank· DDBJ
ACN88562.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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