C5CM11 · RUVA_VARPS
- ProteinHolliday junction branch migration complex subunit RuvA
- GeneruvA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids190 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. HJ branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | Holliday junction helicase complex | |
Cellular Component | Holliday junction resolvase complex | |
Molecular Function | ATP binding | |
Molecular Function | four-way junction DNA binding | |
Molecular Function | four-way junction helicase activity | |
Biological Process | DNA recombination | |
Biological Process | DNA repair |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameHolliday junction branch migration complex subunit RuvA
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Variovorax
Accessions
- Primary accessionC5CM11
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000202006 | 1-190 | Holliday junction branch migration complex subunit RuvA | |||
Sequence: MIGKLTGILAERNPPQVVVDCNGVGYEVDVPMSTFYNLPGTGERVSLLTHFVVREDAQILYGFGTAEERAAFRQLIKISGVGPRTALGLLSGMSVGELSQAITTQELGRLVKIPGIGKKTAERLLLELKGKLGADIGLPAHAATDAQADILQALVALGYSDKEAALALKALPKDATVSEGIKLALKALAK |
Interaction
Subunit
Homotetramer. Forms an RuvA8-RuvB12-Holliday junction (HJ) complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA strand where it exits the tetramer. Each RuvB hexamer is contacted by two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this complex drives branch migration. In the full resolvosome a probable DNA-RuvA4-RuvB12-RuvC2 complex forms which resolves the HJ.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-64 | Domain I | ||||
Sequence: MIGKLTGILAERNPPQVVVDCNGVGYEVDVPMSTFYNLPGTGERVSLLTHFVVREDAQILYGFG | ||||||
Region | 65-137 | Domain II | ||||
Sequence: TAEERAAFRQLIKISGVGPRTALGLLSGMSVGELSQAITTQELGRLVKIPGIGKKTAERLLLELKGKLGADIG | ||||||
Region | 137-141 | Flexible linker | ||||
Sequence: GLPAH | ||||||
Region | 142-190 | Domain III | ||||
Sequence: AATDAQADILQALVALGYSDKEAALALKALPKDATVSEGIKLALKALAK |
Domain
Has three domains with a flexible linker between the domains II and III and assumes an 'L' shape. Domain III is highly mobile and contacts RuvB.
Sequence similarities
Belongs to the RuvA family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length190
- Mass (Da)19,946
- Last updated2009-07-28 v1
- Checksum55BC66A684703A92
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001635 EMBL· GenBank· DDBJ | ACS21270.1 EMBL· GenBank· DDBJ | Genomic DNA |