C0JB89 · B2L1_SICCD
- ProteinDermonecrotic toxin SdSicTox-betaIIB2i
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids274 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Dermonecrotic toxins cleave the phosphodiester linkage between the phosphate and headgroup of certain phospholipids (sphingolipid and lysolipid substrates), forming an alcohol (often choline) and a cyclic phosphate (By similarity).
This toxin acts on sphingomyelin (SM) (By similarity).
It may also act on ceramide phosphoethanolamine (CPE), lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), but not on lysophosphatidylserine (LPS), and lysophosphatidylglycerol (LPG) (By similarity).
It acts by transphosphatidylation, releasing exclusively cyclic phosphate products as second products (By similarity).
Induces dermonecrosis, hemolysis, increased vascular permeability, edema, inflammatory response, and platelet aggregation (By similarity).
This toxin acts on sphingomyelin (SM) (By similarity).
It may also act on ceramide phosphoethanolamine (CPE), lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), but not on lysophosphatidylserine (LPS), and lysophosphatidylglycerol (LPG) (By similarity).
It acts by transphosphatidylation, releasing exclusively cyclic phosphate products as second products (By similarity).
Induces dermonecrosis, hemolysis, increased vascular permeability, edema, inflammatory response, and platelet aggregation (By similarity).
Catalytic activity
- an N-(acyl)-sphingosylphosphocholine = an N-(acyl)-sphingosyl-1,3-cyclic phosphate + choline
- an N-(acyl)-sphingosylphosphoethanolamine = an N-(acyl)-sphingosyl-1,3-cyclic phosphate + ethanolamine
- a 1-acyl-sn-glycero-3-phosphocholine = a 1-acyl-sn-glycero-2,3-cyclic phosphate + choline
- a 1-acyl-sn-glycero-3-phosphoethanolamine = a 1-acyl-sn-glycero-2,3-cyclic phosphate + ethanolamine
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | lyase activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoric diester hydrolase activity | |
Molecular Function | toxin activity | |
Biological Process | killing of cells of another organism | |
Biological Process | lipid catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDermonecrotic toxin SdSicTox-betaIIB2i
- EC number
- Alternative names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Chelicerata > Arachnida > Araneae > Araneomorphae > Haplogynae > Scytodoidea > Sicariidae > Sicarius
Accessions
- Primary accessionC0JB89
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000392901 | 1-274 | Dermonecrotic toxin SdSicTox-betaIIB2i | |||
Sequence: WIMGHMVNGLEQVSEFLNLGANAIEFDIDFDQNGVAKITHHGIPCDCGRLCTKQTVFTEYLDNIRHVTTPGDPKFREQLILLALDLKLQRIPVEKAYAAGVDVATKLLDHYWQRGKSKARAYILLNLPLVQDYEFIRAFKDTLKNEGYEQYNDKVGVNFTGNEDLDEIRKVLKKVGVDKHVWQADGITSCFARGTDRLTEALKRRDTPGYNYAYKVYAWTLVKYSTMRRSLRLGVDGVMSNFPDRVVEVLKEEEFADKFRMATYDDNPWKKFTG | ||||||
Disulfide bond | 45↔51 | |||||
Sequence: CDCGRLC | ||||||
Disulfide bond | 47↔190 | |||||
Sequence: CGRLCTKQTVFTEYLDNIRHVTTPGDPKFREQLILLALDLKLQRIPVEKAYAAGVDVATKLLDHYWQRGKSKARAYILLNLPLVQDYEFIRAFKDTLKNEGYEQYNDKVGVNFTGNEDLDEIRKVLKKVGVDKHVWQADGITSC |
Keywords
- PTM
Expression
Tissue specificity
Expressed by the venom gland.
Structure
Family & Domains
Sequence similarities
Belongs to the arthropod phospholipase D family. Class II subfamily.
Family and domain databases
Sequence
- Sequence statusFragment
- Length274
- Mass (Da)31,562
- Last updated2009-05-05 v1
- Checksum315A623694A03B49
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: W |