B9JJJ9 · MURQ_RHIR8
- ProteinN-acetylmuramic acid 6-phosphate etherase
- GenemurQ
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids307 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.
Miscellaneous
A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.
Catalytic activity
- N-acetyl-D-muramate 6-phosphate + H2O = N-acetyl-D-glucosamine 6-phosphate + (R)-lactate
Pathway
Amino-sugar metabolism; 1,6-anhydro-N-acetylmuramate degradation.
Amino-sugar metabolism; N-acetylmuramate degradation.
Cell wall biogenesis; peptidoglycan recycling.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 90 | Proton donor | ||||
Sequence: E | ||||||
Active site | 121 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carbohydrate derivative binding | |
Molecular Function | carbon-oxygen lyase activity | |
Molecular Function | ether hydrolase activity | |
Biological Process | 1,6-anhydro-N-acetyl-beta-muramic acid catabolic process | |
Biological Process | amino sugar catabolic process | |
Biological Process | N-acetylmuramic acid catabolic process | |
Biological Process | peptidoglycan turnover |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylmuramic acid 6-phosphate etherase
- EC number
- Short namesMurNAc-6-P etherase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Rhizobium/Agrobacterium group > Rhizobium
Accessions
- Primary accessionB9JJJ9
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000118004 | 1-307 | N-acetylmuramic acid 6-phosphate etherase | |||
Sequence: MTEQRLISELEQLVSEGRNPNTMHIDLLPTFDILREINYEDQTVPTAVEKVIPAIAAAVNQIVAAFQKGGRLIYMGAGTSGRLGVLDASECPPTFSVPSDMVIGLIAGGPEALQNSIEGAEDDPEQGRQALQDIKLTSTDVVVGIAVSGRTPYVIGGLNYAKSIGAVTIALSCNPNSIIAGIADLAISPVVGPEILTGSTRLKSGTAQKLILNMLTTASMIRIGKSYQNLMVDVHASNKKLVARAIRIVMQATGCTQADARRVLDQTGNDVKLAILMEITGMGIEEARAALQNAGGFLRKAISAKTA |
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 62-225 | SIS | ||||
Sequence: IVAAFQKGGRLIYMGAGTSGRLGVLDASECPPTFSVPSDMVIGLIAGGPEALQNSIEGAEDDPEQGRQALQDIKLTSTDVVVGIAVSGRTPYVIGGLNYAKSIGAVTIALSCNPNSIIAGIADLAISPVVGPEILTGSTRLKSGTAQKLILNMLTTASMIRIGK |
Sequence similarities
Belongs to the GCKR-like family. MurNAc-6-P etherase subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length307
- Mass (Da)32,321
- Last updated2009-03-24 v1
- Checksum4BFBDE7631B7E833
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000629 EMBL· GenBank· DDBJ | ACM30091.1 EMBL· GenBank· DDBJ | Genomic DNA |