B8NI20 · IMQC_ASPFN

Function

function

FAD-dependent monooxygenase; part of the gene cluster that mediates the biosynthesis of imizoquins A to D, tripeptide-derived alkaloids that serve a protective role against oxidative stress that are essential for normal germination (PubMed:29182847).
ImqB is a canonical three-module NRPS that assembles the tripeptide backbone of the imizoquins via condensation of Trp, Tyr, and Leu-derived precursors (PubMed:29182847).
N-methylation by imqF and phenol oxidation by imqC, followed by cyclization via the FAD-dependent oxidase imqH carry out the three-step transformation of L-tyrosine into tetrahydroisoquinoline (PubMed:29182847).
Importantly, this sequence requires the presence of a free amine in the tyrosine moiety, indicating that isoquinoline formation occurs prior to peptide bond formation (PubMed:29182847).
The imidazolidin-4-one ring of imizoquins could form following additional oxidation of the methyl-derived bridgehead carbon by imqH (PubMed:29182847).
Lastly, O-methylation by imqG and leucine hydroxylation by imqE complete biosynthesis of the imizoquins (PubMed:29182847).

Pathway

Secondary metabolite biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site139-141FAD (UniProtKB | ChEBI)
Binding site189FAD (UniProtKB | ChEBI)
Binding site210FAD (UniProtKB | ChEBI)

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    FAD-dependent monooxygenase imqC
  • EC number
  • Alternative names
    • Imizoquin biosynthesis cluster protein C

Gene names

    • Name
      imqC
    • ORF names
      AFLA_064250

Organism names

Accessions

  • Primary accession
    B8NI20

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004445511-223FAD-dependent monooxygenase imqC

Expression

Induction

Expression is down-regulated by ralstonins, lipopeptides produced by the plant pathogenic bacteria Ralstonia solanacearum (PubMed:29182847).
Expression is positively regulated by the imizoquins cluster-specific transcription regulator imqK (PubMed:29182847).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the PheA/TfdB FAD monooxygenase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    223
  • Mass (Da)
    25,367
  • Last updated
    2009-03-03 v1
  • Checksum
    E5634B522C36BE5B
MIENLRKRSTIEVEWRKQPVHMDIDLDQVDNPEAYPITVSVETSKDGSDPGLLWQPMHAERDSIVKETIHAKYVLGCDGARSWIRQRLGVSFIGDLTDSTWGVMDIVPKTSFPDIRKVAVIHSSKGTVMSVPREDKLVRFYIQIDAVNPNAASGLARRDLKVEDLLDAARAIMFPYTMEAAECAWWSAYRVGQRVANEFARHDRIFLAGDSVREYCLEVIRCQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EQ963479
EMBL· GenBank· DDBJ
EED49604.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp