B8D4I6 · THII_DESA1
- ProteintRNA sulfurtransferase
- GenethiI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids482 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.
Catalytic activity
- [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + ATP + H+ = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + 2 oxidized [2Fe-2S]-[ferredoxin] + AMP + diphosphate
- [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + L-cysteinyl-[cysteine desulfurase] + A + AMP + H+
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 178-179 | ATP (UniProtKB | ChEBI) | ||||
Sequence: LF | ||||||
Binding site | 203-204 | ATP (UniProtKB | ChEBI) | ||||
Sequence: HY | ||||||
Binding site | 260 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 282 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 291 | ATP (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Active site | 444 | Cysteine persulfide intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | CCA tRNA nucleotidyltransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uracil-4 sulfurtransferase activity | |
Biological Process | thiamine biosynthetic process | |
Biological Process | thiamine diphosphate biosynthetic process | |
Biological Process | thiazole biosynthetic process | |
Biological Process | tRNA 4-thiouridine biosynthesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA sulfurtransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Desulfurococcales > Desulfurococcaceae > Desulfurococcus
Accessions
- Primary accessionB8D4I6
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000196940 | 1-482 | tRNA sulfurtransferase | |||
Sequence: MPTYLITVAGEIPLKSKKTRSRLYYRLIDNIRRRLARRNITLQVAKVIDAKILVETQVEALQELSRVFGVHRVSEVQVLEFRDLGELAKEIASRTIEHVRDRKFAVRVKRSGRHGFTSLDVAREVGALLKPYSKGVDLENPDIEVEVEVRGNKAYLYSNVAMGPGGLPLGSSGRALVLFSGGFDSPVAAWMIAKRGVEVDFLHYVMGSSEVSRQAFSVARKLSEEWLSSYNPRFITVDFTPLIAEIEERIEWSYRQVVLRALMYMVADKIATELGYNTIVTGEALSQASSQTLANLVAVESAVSPRSIILRPLIGFDKEEIIEYSRRIGLYDYSSRVAETCAIAPTHVVTRISSEKLKSLIERLDVRLVERMAGEYRVVDVFSASPEEAVPGYSEEIDSIPGDSIIIDVRSYEEYKRDALPGAIHLSMVDFNNLPRDKPVVLYCTTGGISLLLARELRGKGFKAYSLRGGLARYRAGLEKTR | ||||||
Disulfide bond | 341↔444 | Redox-active | ||||
Sequence: CAIAPTHVVTRISSEKLKSLIERLDVRLVERMAGEYRVVDVFSASPEEAVPGYSEEIDSIPGDSIIIDVRSYEEYKRDALPGAIHLSMVDFNNLPRDKPVVLYC |
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 58-160 | THUMP | ||||
Sequence: VEALQELSRVFGVHRVSEVQVLEFRDLGELAKEIASRTIEHVRDRKFAVRVKRSGRHGFTSLDVAREVGALLKPYSKGVDLENPDIEVEVEVRGNKAYLYSNV | ||||||
Domain | 400-482 | Rhodanese | ||||
Sequence: IPGDSIIIDVRSYEEYKRDALPGAIHLSMVDFNNLPRDKPVVLYCTTGGISLLLARELRGKGFKAYSLRGGLARYRAGLEKTR |
Sequence similarities
Belongs to the ThiI family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length482
- Mass (Da)53,985
- Last updated2009-03-03 v1
- Checksum0800B22D46C77A5F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001140 EMBL· GenBank· DDBJ | ACL11017.1 EMBL· GenBank· DDBJ | Genomic DNA |