B8D4I6 · THII_DESA1

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site178-179ATP (UniProtKB | ChEBI)
Binding site203-204ATP (UniProtKB | ChEBI)
Binding site260ATP (UniProtKB | ChEBI)
Binding site282ATP (UniProtKB | ChEBI)
Binding site291ATP (UniProtKB | ChEBI)
Active site444Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA 4-thiouridine biosynthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • Ordered locus names
      DKAM_0691

Organism names

Accessions

  • Primary accession
    B8D4I6

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_10001969401-482tRNA sulfurtransferase
Disulfide bond341↔444Redox-active

Keywords

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain58-160THUMP
Domain400-482Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    482
  • Mass (Da)
    53,985
  • Last updated
    2009-03-03 v1
  • Checksum
    0800B22D46C77A5F
MPTYLITVAGEIPLKSKKTRSRLYYRLIDNIRRRLARRNITLQVAKVIDAKILVETQVEALQELSRVFGVHRVSEVQVLEFRDLGELAKEIASRTIEHVRDRKFAVRVKRSGRHGFTSLDVAREVGALLKPYSKGVDLENPDIEVEVEVRGNKAYLYSNVAMGPGGLPLGSSGRALVLFSGGFDSPVAAWMIAKRGVEVDFLHYVMGSSEVSRQAFSVARKLSEEWLSSYNPRFITVDFTPLIAEIEERIEWSYRQVVLRALMYMVADKIATELGYNTIVTGEALSQASSQTLANLVAVESAVSPRSIILRPLIGFDKEEIIEYSRRIGLYDYSSRVAETCAIAPTHVVTRISSEKLKSLIERLDVRLVERMAGEYRVVDVFSASPEEAVPGYSEEIDSIPGDSIIIDVRSYEEYKRDALPGAIHLSMVDFNNLPRDKPVVLYCTTGGISLLLARELRGKGFKAYSLRGGLARYRAGLEKTR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001140
EMBL· GenBank· DDBJ
ACL11017.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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