B7NL80 · TRHO_ECO7I
- ProteintRNA uridine(34) hydroxylase
- GenetrhO
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids350 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes oxygen-dependent 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs, the first step in 5-carboxymethoxyuridine (cmo5U) biosynthesis. May be part of an alternate pathway, which is able to bypass cmo5U biogenesis in a subset of tRNAs under aerobic conditions.
Catalytic activity
- AH2 + O2 + uridine34 in tRNA = 5-hydroxyuridine34 in tRNA + A + H2O
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 200 | Cysteine persulfide intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA uridine(34) hydroxylase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionB7NL80
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000200359 | 1-350 | tRNA uridine34 hydroxylase | |||
Sequence: MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDALYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDDGKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 146-240 | Rhodanese | ||||
Sequence: DDPDALFIDMRNHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGL |
Sequence similarities
Belongs to the TrhO family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length350
- Mass (Da)39,735
- Last updated2009-03-24 v1
- Checksum61AB5F317B0C24DB
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CU928164 EMBL· GenBank· DDBJ | CAR18236.1 EMBL· GenBank· DDBJ | Genomic DNA |