B7N4P2 · MAO1_ECOLU
- ProteinNAD-dependent malic enzyme
- GenemaeA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids565 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
Catalytic activity
- (S)-malate + NAD+ = CO2 + NADH + pyruvate
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Divalent metal cations. Prefers magnesium or manganese.
Features
Showing features for active site, binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 104 | Proton donor | ||||
Sequence: Y | ||||||
Binding site | 157 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 175 | Proton acceptor | ||||
Sequence: K | ||||||
Binding site | 246 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 247 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 270 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 270 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Site | 270 | Important for activity | ||||
Sequence: D | ||||||
Binding site | 418 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | malate dehydrogenase (decarboxylating) (NAD+) activity | |
Molecular Function | metal ion binding | |
Molecular Function | NAD binding | |
Molecular Function | oxaloacetate decarboxylase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNAD-dependent malic enzyme
- EC number
- Short namesNAD-ME
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionB7N4P2
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000185996 | 1-565 | NAD-dependent malic enzyme | |||
Sequence: MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length565
- Mass (Da)63,163
- Last updated2009-03-24 v1
- ChecksumFFD2C913F7028F3A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CU928163 EMBL· GenBank· DDBJ | CAR12936.1 EMBL· GenBank· DDBJ | Genomic DNA |