B4Q068 · NO66_DROYA

  • Protein
    Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code (By similarity).

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Features

Showing features for binding site.

168350100150200250300350400450500550600650
TypeIDPosition(s)Description
Binding site381Fe cation (UniProtKB | ChEBI); catalytic
Binding site383Fe cation (UniProtKB | ChEBI); catalytic
Binding site446Fe cation (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentnucleolus
Cellular Componentnucleus
Molecular Function2-oxoglutarate-dependent dioxygenase activity
Molecular Functionhistone H3K36 demethylase activity
Molecular Functionhistone H3K36me/H3K36me2 demethylase activity
Molecular Functionhistone H3K4me/H3K4me2/H3K4me3 demethylase activity
Molecular Functioniron ion binding
Biological Processnegative regulation of DNA-templated transcription

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
  • EC number
  • Alternative names
    • Histone lysine demethylase NO66

Gene names

    • ORF names
      GE16340

Organism names

  • Taxonomic identifier
  • Strain
    • Tai18E2 / Tucson 14021-0261.01
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora

Accessions

  • Primary accession
    B4Q068

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00003909941-683Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
Modified residue152Phosphoserine
Modified residue158Phosphothreonine
Modified residue159Phosphoserine

Keywords

Structure

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias1-33Polar residues
Region1-162Disordered
Compositional bias54-82Basic and acidic residues
Compositional bias83-97Polar residues
Compositional bias115-147Polar residues
Region179-208Disordered
Domain341-480JmjC

Sequence similarities

Belongs to the ROX family. NO66 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    683
  • Mass (Da)
    76,493
  • Last updated
    2008-09-23 v1
  • Checksum
    841872F89FC74CF1
MHKASTSSANRANFQGNHKTQKSPNNGKAKAKKSPNTDINVADVEMLLNPRSNLTKEQKERRKMMEGFVTKTFERAENESDGSDIDTSASTSNKGKSKAARPTDRKRRLQAEDSPPADANNNNTKNGKDGKVSKESASTQGASATKRKPPRSQGLEHTSPIQVDGEALACPLVRKSLPAAGASGASGPAKSCPLPEKRKSLPAGAAKSKSQVIKQEALEEASNEAQLLALPIETHKTDSIEEGRRVLQWLLNPIKVNHFFDDFWEHTAFVVQRKNPHYYSKLISFKMIDEMLVRHRLDFTINVDVTTYKNGKRETLNPEGRALPPVVWGLYSEGCSIRILNPSTYLVGLRQVCSIMQEFFHCLVGANVYLTPPNSQGFAPHYDDIEAFVIQVEGRKRWRLYEPPSGSDQLCRNSSSNFDQEQLGEPILDEVLEAGDLLYFPRGTVHQAITEEEQHSLHITLSVYQQQAYVNLLEKLMPIVLKKAIKQSVALRRGLPLHTFHVLGEAQRANRSDSRNQLVENVQKLVTKHLMPSAQDIDEAVDQLAKKFQHEALPPIILPEEQVRTVFGSRSTADEQGNAICDYEFDTKTSVRLLRANILRLVTEEDGSVRIYHHVDNGFEYCKYEPIFMEILPEEAAAVELLISAYPYYLTVGQMPLDSAARKVEVVTALWERGLLMTEKPFK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-33Polar residues
Compositional bias54-82Basic and acidic residues
Compositional bias83-97Polar residues
Compositional bias115-147Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM000162
EMBL· GenBank· DDBJ
EDX01220.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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