B4Q068 · NO66_DROYA
- ProteinBifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids683 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code (By similarity).
Catalytic activity
- 2 2-oxoglutarate + N6,N6-dimethyl-L-lysyl36-[histone H3] + 2 O2 = 2 CO2 + 2 formaldehyde + L-lysyl36-[histone H3] + 2 succinate
2 CHEBI:16810 + RHEA-COMP:9787 CHEBI:61976 Position: 36+ 2 CHEBI:15379 = 2 CHEBI:16526 + 2 CHEBI:16842 + RHEA-COMP:9785 CHEBI:29969 Position: 36+ 2 CHEBI:30031
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleolus | |
Cellular Component | nucleus | |
Molecular Function | 2-oxoglutarate-dependent dioxygenase activity | |
Molecular Function | histone H3K36 demethylase activity | |
Molecular Function | histone H3K36me/H3K36me2 demethylase activity | |
Molecular Function | histone H3K4me/H3K4me2/H3K4me3 demethylase activity | |
Molecular Function | iron ion binding | |
Biological Process | negative regulation of DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameBifunctional lysine-specific demethylase and histidyl-hydroxylase NO66
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionB4Q068
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000390994 | 1-683 | Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 | |||
Sequence: MHKASTSSANRANFQGNHKTQKSPNNGKAKAKKSPNTDINVADVEMLLNPRSNLTKEQKERRKMMEGFVTKTFERAENESDGSDIDTSASTSNKGKSKAARPTDRKRRLQAEDSPPADANNNNTKNGKDGKVSKESASTQGASATKRKPPRSQGLEHTSPIQVDGEALACPLVRKSLPAAGASGASGPAKSCPLPEKRKSLPAGAAKSKSQVIKQEALEEASNEAQLLALPIETHKTDSIEEGRRVLQWLLNPIKVNHFFDDFWEHTAFVVQRKNPHYYSKLISFKMIDEMLVRHRLDFTINVDVTTYKNGKRETLNPEGRALPPVVWGLYSEGCSIRILNPSTYLVGLRQVCSIMQEFFHCLVGANVYLTPPNSQGFAPHYDDIEAFVIQVEGRKRWRLYEPPSGSDQLCRNSSSNFDQEQLGEPILDEVLEAGDLLYFPRGTVHQAITEEEQHSLHITLSVYQQQAYVNLLEKLMPIVLKKAIKQSVALRRGLPLHTFHVLGEAQRANRSDSRNQLVENVQKLVTKHLMPSAQDIDEAVDQLAKKFQHEALPPIILPEEQVRTVFGSRSTADEQGNAICDYEFDTKTSVRLLRANILRLVTEEDGSVRIYHHVDNGFEYCKYEPIFMEILPEEAAAVELLISAYPYYLTVGQMPLDSAARKVEVVTALWERGLLMTEKPFK | ||||||
Modified residue | 152 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 158 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 159 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-33 | Polar residues | ||||
Sequence: MHKASTSSANRANFQGNHKTQKSPNNGKAKAKK | ||||||
Region | 1-162 | Disordered | ||||
Sequence: MHKASTSSANRANFQGNHKTQKSPNNGKAKAKKSPNTDINVADVEMLLNPRSNLTKEQKERRKMMEGFVTKTFERAENESDGSDIDTSASTSNKGKSKAARPTDRKRRLQAEDSPPADANNNNTKNGKDGKVSKESASTQGASATKRKPPRSQGLEHTSPIQ | ||||||
Compositional bias | 54-82 | Basic and acidic residues | ||||
Sequence: LTKEQKERRKMMEGFVTKTFERAENESDG | ||||||
Compositional bias | 83-97 | Polar residues | ||||
Sequence: SDIDTSASTSNKGKS | ||||||
Compositional bias | 115-147 | Polar residues | ||||
Sequence: PPADANNNNTKNGKDGKVSKESASTQGASATKR | ||||||
Region | 179-208 | Disordered | ||||
Sequence: AAGASGASGPAKSCPLPEKRKSLPAGAAKS | ||||||
Domain | 341-480 | JmjC | ||||
Sequence: NPSTYLVGLRQVCSIMQEFFHCLVGANVYLTPPNSQGFAPHYDDIEAFVIQVEGRKRWRLYEPPSGSDQLCRNSSSNFDQEQLGEPILDEVLEAGDLLYFPRGTVHQAITEEEQHSLHITLSVYQQQAYVNLLEKLMPIV |
Sequence similarities
Belongs to the ROX family. NO66 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length683
- Mass (Da)76,493
- Last updated2008-09-23 v1
- Checksum841872F89FC74CF1
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-33 | Polar residues | ||||
Sequence: MHKASTSSANRANFQGNHKTQKSPNNGKAKAKK | ||||||
Compositional bias | 54-82 | Basic and acidic residues | ||||
Sequence: LTKEQKERRKMMEGFVTKTFERAENESDG | ||||||
Compositional bias | 83-97 | Polar residues | ||||
Sequence: SDIDTSASTSNKGKS | ||||||
Compositional bias | 115-147 | Polar residues | ||||
Sequence: PPADANNNNTKNGKDGKVSKESASTQGASATKR |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM000162 EMBL· GenBank· DDBJ | EDX01220.1 EMBL· GenBank· DDBJ | Genomic DNA |