B3FHT4 · SUCA_BLAHN

Function

function

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.

Catalytic activity

Kinetics

kcat is 133 min-1 with ATP as substrate.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site38-41CoA (UniProtKB | ChEBI)
Binding site64CoA (UniProtKB | ChEBI)
Binding site117-119CoA (UniProtKB | ChEBI)
Binding site181substrate; ligand shared with subunit beta
Active site271Tele-phosphohistidine intermediate

GO annotations

AspectTerm
Cellular Componenthydrogenosome
Cellular Componentmitochondrion
Cellular Componentsuccinate-CoA ligase complex (ADP-forming)
Molecular Functionnucleotide binding
Molecular Functionsuccinate-CoA ligase (ADP-forming) activity
Molecular Functionsuccinate-CoA ligase (GDP-forming) activity
Biological Processtricarboxylic acid cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Succinate--CoA ligase [ADP-forming] subunit alpha
  • EC number
  • Alternative names
    • Succinyl-CoA synthetase subunit alpha
      (SCS-alpha
      )

Gene names

    • Name
      SCSa

Organism names

Accessions

  • Primary accession
    B3FHT4
  • Secondary accessions
    • B3FHN9

Proteomes

Subcellular Location

Hydrogenosome
Note: Found in the matrix of the mitochondrion-like organelles (MLO) of Blastocystis.

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-18Hydrogenosome
ChainPRO_000043859419-318Succinate--CoA ligase [ADP-forming] subunit alpha

Interaction

Subunit

Heterodimer of an alpha and a beta subunit.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the succinate/malate CoA ligase alpha subunit family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    318
  • Mass (Da)
    33,380
  • Last updated
    2008-07-22 v1
  • Checksum
    9BAC52317C9237E1
MLSRVSQVSRVGFSLARASSTARVWVDKNTRVIGQGITGKNGTFHTQQAIEYGTKMVGGVNPKKAGTTHLGLPIFASVEEAKKATNADATVIYVPPSGAAGAILEAVAAEIPLIVCITEGIPQHDMLRVKDIMKSQNKSRLIGPNCPGIIKPEECKIGIMPGYIHKKGKIGIVSRSGTLTYEAVHQTTMYGLGQSTVVGIGGDPFNGTNFIDCLERFTNDPQTEGIVMIGEIGGTAEEDAAEWLKQYGNPNKPVVSFIAGRTAPPGRRMGHAGAIISGGKGTAKAKMEALTSAGVKVVETPAMIGEAMFNMMKAAGKA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EU076378
EMBL· GenBank· DDBJ
ABY62723.1
EMBL· GenBank· DDBJ
mRNA
EU090061
EMBL· GenBank· DDBJ
ABW76150.1
EMBL· GenBank· DDBJ
Genomic DNA
LXWW01000228
EMBL· GenBank· DDBJ
OAO14631.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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