B3FHT4 · SUCA_BLAHN
- ProteinSuccinate--CoA ligase [ADP-forming] subunit alpha
- GeneSCSa
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids318 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.
Catalytic activity
- succinate + ATP + CoA = succinyl-CoA + ADP + phosphate
Kinetics
kcat is 133 min-1 with ATP as substrate.
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 38-41 | CoA (UniProtKB | ChEBI) | ||||
Sequence: TGKN | ||||||
Binding site | 64 | CoA (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 117-119 | CoA (UniProtKB | ChEBI) | ||||
Sequence: ITE | ||||||
Binding site | 181 | substrate; ligand shared with subunit beta | ||||
Sequence: Y | ||||||
Active site | 271 | Tele-phosphohistidine intermediate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | hydrogenosome | |
Cellular Component | mitochondrion | |
Cellular Component | succinate-CoA ligase complex (ADP-forming) | |
Molecular Function | nucleotide binding | |
Molecular Function | succinate-CoA ligase (ADP-forming) activity | |
Molecular Function | succinate-CoA ligase (GDP-forming) activity | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSuccinate--CoA ligase [ADP-forming] subunit alpha
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Sar > Stramenopiles > Bigyra > Opalozoa > Opalinata > Blastocystidae > Blastocystis
Accessions
- Primary accessionB3FHT4
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Found in the matrix of the mitochondrion-like organelles (MLO) of Blastocystis.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-18 | Hydrogenosome | ||||
Sequence: MLSRVSQVSRVGFSLARA | ||||||
Chain | PRO_0000438594 | 19-318 | Succinate--CoA ligase [ADP-forming] subunit alpha | |||
Sequence: SSTARVWVDKNTRVIGQGITGKNGTFHTQQAIEYGTKMVGGVNPKKAGTTHLGLPIFASVEEAKKATNADATVIYVPPSGAAGAILEAVAAEIPLIVCITEGIPQHDMLRVKDIMKSQNKSRLIGPNCPGIIKPEECKIGIMPGYIHKKGKIGIVSRSGTLTYEAVHQTTMYGLGQSTVVGIGGDPFNGTNFIDCLERFTNDPQTEGIVMIGEIGGTAEEDAAEWLKQYGNPNKPVVSFIAGRTAPPGRRMGHAGAIISGGKGTAKAKMEALTSAGVKVVETPAMIGEAMFNMMKAAGKA |
Interaction
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length318
- Mass (Da)33,380
- Last updated2008-07-22 v1
- Checksum9BAC52317C9237E1
Keywords
- Technical term