B3EWR5 · HEXL_LUPAL

Function

function

Has hexosaminidase activity. Active with both p-nitrophenyl-beta-D-N-acetylglucosamine (pNP-GlcNAc) and p-nitrophenyl-beta-D-N-acetylgalactosamine (pNP-GalNAc). Not active toward p-nitrophenyl-beta-D-N,N'-diacetylchitobiose (pNP-(GlcNAc)2) or p-nitrophenyl-beta-D-N,N',N''-triacetylchitobiose (pNP-(GlcNAc)3). Removes terminal GlcNAc and may be involved in storage protein degradation.

Catalytic activity

  • Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
    EC:3.2.1.52 (UniProtKB | ENZYME | Rhea)

Activity regulation

Inhibited by AgNO3 at a concentration of 0.1 mM. Strongly inhibited by CdCl2, ZnCl2 and FeCl3 and moderately by CoCl2, CuSO4 and NiCl2 at 10 mM concentration. CaCl2, MgCl2, MnSO4 and KI also have a slight inhibitory effect of 20%-25% at 10 mM concentration. Activated to a small extent by MgCl2 at 0.1 mM concentration but inhibited with increasing concentration. Not affected by carbohydrates such as fucose, galactose and glucose but displays a slight decrease in activity up to 25% with lactose, alpha-mannose and N-acetyl-galactosamine (GalNAc).

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
2.59 mMpNP-GlcNAc
2.94 mMpNP-GalcNAc
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
18.4 μmol/min/mgwith pNP-GlcNAc as substrate
2.73 μmol/min/mgwith pNP-GalcNAc as substrate

pH Dependence

Optimum pH is 5.0 with pNP-GlcNAc and 4.0 with pNP-GalNAc. Half maximal activity observed at pH 3.0 and 6.5 with pNP-GlcNAc and at pH 2.5 and 5.5 with pNP-GalNAc. Retains over 90% of its activity between pH 6.5-8.0 with pNP-GlcNAc, and between pH 6.0-7.5 with pNP-GalNAc.

Temperature Dependence

Optimum temperature is 50 degrees Celsius toward pNP-GlcNAc and 60 degrees Celsius toward pNP-GalNAc. Displays activity higher than 50% between 35-70 degrees Celsius. Stable under 30 degrees Celsius.

GO annotations

AspectTerm
Molecular Functionbeta-N-acetylhexosaminidase activity
Molecular FunctionN-acetyl-beta-D-galactosaminidase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Beta-hexosaminidase
  • EC number
  • Alternative names
    • Beta-GlcNAcase
    • Beta-N-acetylhexosaminidase (Beta-NAHase)
    • N-acetyl-beta-glucosaminidase

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > genistoids sensu lato > core genistoids > Genisteae > Lupinus

Accessions

  • Primary accession
    B3EWR5

PTM/Processing

Features

Showing features for peptide.

TypeIDPosition(s)Description
PeptidePRO_00004225561-26Beta-hexosaminidase

Post-translational modification

Glycosylated.

Keywords

Expression

Tissue specificity

Detected in dry seeds and cotyledons.

Developmental stage

Activity levels increase 5-9 days after imbibition in the hypocotyls and within 3-9 days in the roots. Detected in dry seeds but increased levels observed 5 days after seed imbibition.

Family & Domains

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    26
  • Mass (Da)
    2,980
  • Last updated
    2013-11-13 v2
  • Checksum
    5F04E3EC26FC875D
VDSEDLIEAFKIYVEDDNEHLQGSVD

Features

Showing features for sequence uncertainty, non-terminal residue.

TypeIDPosition(s)Description
Sequence uncertainty8E or N
Non-terminal residue26

Keywords

Similar Proteins

Disclaimer

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