B1LWN4 · LIPA_METRJ

Function

function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N6-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site64[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site69[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site75[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site90[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site94[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site97[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site303[4Fe-4S] cluster 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionlipoate synthase activity
Molecular Functionmetal ion binding
Biological Processprotein lipoylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lipoyl synthase
  • EC number
  • Alternative names
    • Lip-syn
      (LS
      )
    • Lipoate synthase
    • Lipoic acid synthase
    • Sulfur insertion protein LipA

Gene names

    • Name
      lipA
    • Ordered locus names
      Mrad2831_2176

Organism names

Accessions

  • Primary accession
    B1LWN4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000996131-327Lipoyl synthase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-32Disordered
Compositional bias11-32Basic and acidic residues
Domain76-292Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    327
  • Mass (Da)
    36,245
  • Last updated
    2008-04-29 v1
  • Checksum
    AA09B4FF34385F53
MAVVLDTLKNDPRPVRLRHPEKAHRPDQPVQAKKPDWIRVKAPGSRAWSETQKIVREHGLVTVCEEAGCPNIGECWEKRHATFMIMGDTCTRACAFCNVRTGLPDALDLGEPDKIADSVAKLGLHHVVITSVDRDDLRDGGAEHFARTIAAIRRASPGTTVEILTPDFLRKDGALEVVVAAKPDVFNHNLETVPAKYLTVRPGARYFHSVRLLQRVKELDPAIFTKSGIMVGLGEERNEVLQLMDDLRSADVDFLTIGQYLQPSKKHHEVVRFVPPDEFKAYETTAYAKGFLLVSATPLTRSSHHAGEDFARLQAARLAKLSPALSA

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias11-32Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP001001
EMBL· GenBank· DDBJ
ACB24171.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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