A9V767 · DRE2_MONBE

Function

function

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the cytosolic Fe-S scaffold complex. Electrons are transferred from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Together with the diflavin oxidoreductase, also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit.

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Features

Showing features for binding site.

128220406080100120140160180200220240260280
TypeIDPosition(s)Description
Binding site203[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site214[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site217[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site219[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site243[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site246[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site254[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site257[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentmitochondrial intermembrane space
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionelectron transfer activity
Molecular Functionmetal ion binding
Biological Processiron-sulfur cluster assembly

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Anamorsin homolog
  • Alternative names
    • Fe-S cluster assembly protein DRE2 homolog

Gene names

    • ORF names
      33738

Organism names

Accessions

  • Primary accession
    A9V767

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003924121-282Anamorsin homolog

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, motif.

TypeIDPosition(s)Description
Region1-140N-terminal SAM-like domain
Region141-192Linker
Region203-219Fe-S binding site A
Motif243-246Cx2C motif 1
Region243-257Fe-S binding site B
Motif254-257Cx2C motif 2

Domain

The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.
The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.
The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.

Sequence similarities

Belongs to the anamorsin family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    282
  • Mass (Da)
    29,474
  • Last updated
    2008-02-05 v1
  • Checksum
    211E2F7841C48461
MADLQGKAVLTIAVPEAKADQIEAEILRLRAATTEAGSVQLEQFDRLEQVFLAPSSYDVIFSGHIALPAKSHADSALAKLAAALKPGGRLALRESLNSRNETALRSALTMGGFVNVQVSTSEHALEAHADKPVYEVGAAAPLKLSFAKKKQSGAAAPAAQVAEVWTIATDDFDDDDLLENDGDELLDAEDLALATTAPEGDDCEVGAGGKRRACKNCTCGRADAEAEQAAKPTLTGPLPASSCGNCYLGDAFRCASCPYLGMPAFKPGEKVTLSDRQLKADA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CH991564
EMBL· GenBank· DDBJ
EDQ86667.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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