A6WR52 · GLYA_SHEB8
- ProteinSerine hydroxymethyltransferase
- GeneglyA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids417 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
Cofactor
Pathway
One-carbon metabolism; tetrahydrofolate interconversion.
Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 121 | (6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 125-127 | (6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: GHL | ||||||
Site | 228 | Plays an important role in substrate specificity | ||||
Sequence: H | ||||||
Binding site | 355-357 | (6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: SPF |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | cobalt ion binding | |
Molecular Function | glycine hydroxymethyltransferase activity | |
Molecular Function | pyridoxal phosphate binding | |
Molecular Function | serine binding | |
Molecular Function | zinc ion binding | |
Biological Process | folic acid metabolic process | |
Biological Process | glycine biosynthetic process from serine | |
Biological Process | L-serine catabolic process | |
Biological Process | tetrahydrofolate interconversion |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine hydroxymethyltransferase
- EC number
- Short namesSHMT ; Serine methylase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Alteromonadales > Shewanellaceae > Shewanella
Accessions
- Primary accessionA6WR52
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000006314 | 1-417 | Serine hydroxymethyltransferase | |||
Sequence: MLKKDMNIADYDPELFKAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIERAKELFSATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYDEMERLAVEHKPKMMIGGFSAYSGIVDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVILSAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVEVFLERGYKIVSGGTSNHLMLVDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEAESKELTGWICDILDDANNPAVIERVKGQVLALCARFPVYG | ||||||
Modified residue | 229 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length417
- Mass (Da)45,237
- Last updated2007-08-21 v1
- Checksum92D3C2F2AB291BDA
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000753 EMBL· GenBank· DDBJ | ABS09291.1 EMBL· GenBank· DDBJ | Genomic DNA |