A6S8P6 · ATG3_BOTFB

Function

function

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of atg8. The atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of atg8 from atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of atg8. The formation of the atg8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The atg8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).

Features

Showing features for active site.

136350100150200250300350
TypeIDPosition(s)Description
Active site247Glycyl thioester intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionAtg8-family conjugating enzyme activity
Biological Processautophagy
Biological Processprotein transport

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Autophagy-related protein 3
  • Alternative names
    • Autophagy-related E2-like conjugation enzyme atg3

Gene names

    • Name
      atg3
    • ORF names
      BC1G_08793, BCIN_08g04530

Organism names

Accessions

  • Primary accession
    A6S8P6
  • Secondary accessions
    • A0A384JQE2

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003178201-363

Interaction

Subunit

Monomer. Interacts with atg8 through an intermediate thioester bond through the C-terminal Gly of atg8. Also interacts with the 40 amino acid C-terminal region of the E1-like atg7 enzyme. Interacts also with the atg12-atg5 conjugate.

Structure

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias84-115Basic and acidic residues
Region84-171Flexible region
Region84-174Disordered
Compositional bias143-166Acidic residues
Region251-339Handle region

Domain

The N-terminal region is involved in phosphatidylethanolamine-binding and is required for atg8-PE conjugation.
The flexible region (FR) is required for atg7-binding.
The handle region (HR) contains the atg8 interaction motif (AIM) and mediates binding to atg8. It is crucial for the cytoplasm-to-vacuole targeting pathway (By similarity).

Sequence similarities

Belongs to the ATG3 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    363
  • Mass (Da)
    40,768
  • Last updated
    2024-05-29 v2
  • Checksum
    27AE827CCDAD925C
MNFLHSTLDRLREFTPVSNTSTFRTNGQITPEEFVAAGDYLVFKFPTWSWADASPTSKRANYLPAGKQFLVTRGVPCHRRLDDDFAGDAGHDETVVRDGEDFRGDGPHSPGDDEDGWLRTGGLAASQEARVRDVRTVDESGEMGEREDDEDDIPDMEDDDDDDEAIIRDPKADNASSSRRTYTIYIAYTPYYRTPRLYLSGYLSSSQPLPPHLMMEDIVGDYKDKTVTLEDFPYFSNNIKMASIHPCKHASVMKTLLDRADAALKLRREKQRQGKAVPGSKDTGMEGLVDDFEKTKIGDKKAVLEGLKAGGNGNDEWEVLQHDQDFANEEEEVAIRVDQYLVVFLKFMASVTPGIEHDFTMGV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias84-115Basic and acidic residues
Compositional bias143-166Acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP009812
EMBL· GenBank· DDBJ
ATZ52825.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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