A6S8P6 · ATG3_BOTFB
- ProteinAutophagy-related protein 3
- Geneatg3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids363 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of atg8. The atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of atg8 from atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of atg8. The formation of the atg8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The atg8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 247 | Glycyl thioester intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | Atg8-family conjugating enzyme activity | |
Biological Process | autophagy | |
Biological Process | protein transport |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameAutophagy-related protein 3
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Leotiomycetes > Helotiales > Sclerotiniaceae > Botrytis
Accessions
- Primary accessionA6S8P6
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Miscellaneous
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000317820 | 1-363 | Autophagy-related protein 3 | |||
Sequence: MNFLHSTLDRLREFTPVSNTSTFRTNGQITPEEFVAAGDYLVFKFPTWSWADASPTSKRANYLPAGKQFLVTRGVPCHRRLDDDFAGDAGHDETVVRDGEDFRGDGPHSPGDDEDGWLRTGGLAASQEARVRDVRTVDESGEMGEREDDEDDIPDMEDDDDDDEAIIRDPKADNASSSRRTYTIYIAYTPYYRTPRLYLSGYLSSSQPLPPHLMMEDIVGDYKDKTVTLEDFPYFSNNIKMASIHPCKHASVMKTLLDRADAALKLRREKQRQGKAVPGSKDTGMEGLVDDFEKTKIGDKKAVLEGLKAGGNGNDEWEVLQHDQDFANEEEEVAIRVDQYLVVFLKFMASVTPGIEHDFTMGV |
Interaction
Subunit
Monomer. Interacts with atg8 through an intermediate thioester bond through the C-terminal Gly of atg8. Also interacts with the 40 amino acid C-terminal region of the E1-like atg7 enzyme. Interacts also with the atg12-atg5 conjugate.
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 84-115 | Basic and acidic residues | ||||
Sequence: DFAGDAGHDETVVRDGEDFRGDGPHSPGDDED | ||||||
Region | 84-171 | Flexible region | ||||
Sequence: DFAGDAGHDETVVRDGEDFRGDGPHSPGDDEDGWLRTGGLAASQEARVRDVRTVDESGEMGEREDDEDDIPDMEDDDDDDEAIIRDPK | ||||||
Region | 84-174 | Disordered | ||||
Sequence: DFAGDAGHDETVVRDGEDFRGDGPHSPGDDEDGWLRTGGLAASQEARVRDVRTVDESGEMGEREDDEDDIPDMEDDDDDDEAIIRDPKADN | ||||||
Compositional bias | 143-166 | Acidic residues | ||||
Sequence: MGEREDDEDDIPDMEDDDDDDEAI | ||||||
Region | 251-339 | Handle region | ||||
Sequence: SVMKTLLDRADAALKLRREKQRQGKAVPGSKDTGMEGLVDDFEKTKIGDKKAVLEGLKAGGNGNDEWEVLQHDQDFANEEEEVAIRVDQ |
Domain
The N-terminal region is involved in phosphatidylethanolamine-binding and is required for atg8-PE conjugation.
The flexible region (FR) is required for atg7-binding.
The handle region (HR) contains the atg8 interaction motif (AIM) and mediates binding to atg8. It is crucial for the cytoplasm-to-vacuole targeting pathway (By similarity).
Sequence similarities
Belongs to the ATG3 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length363
- Mass (Da)40,768
- Last updated2024-05-29 v2
- Checksum27AE827CCDAD925C
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 84-115 | Basic and acidic residues | ||||
Sequence: DFAGDAGHDETVVRDGEDFRGDGPHSPGDDED | ||||||
Compositional bias | 143-166 | Acidic residues | ||||
Sequence: MGEREDDEDDIPDMEDDDDDDEAI |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP009812 EMBL· GenBank· DDBJ | ATZ52825.1 EMBL· GenBank· DDBJ | Genomic DNA |