A3MA34 · RISB_ACIBT
- Protein6,7-dimethyl-8-ribityllumazine synthase
- GeneribH
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids156 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.
Catalytic activity
- (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H+ + 2 H2O + phosphate
Pathway
Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 25 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 59-61 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | ||||
Sequence: AWE | ||||||
Binding site | 83-85 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | ||||
Sequence: AVI | ||||||
Binding site | 88-89 | (2S)-2-hydroxy-3-oxobutyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: ST | ||||||
Active site | 91 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 116 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 130 | (2S)-2-hydroxy-3-oxobutyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | riboflavin synthase complex | |
Molecular Function | 6,7-dimethyl-8-ribityllumazine synthase activity | |
Biological Process | riboflavin biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name6,7-dimethyl-8-ribityllumazine synthase
- EC number
- Short namesDMRL synthase ; LS ; Lumazine synthase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Moraxellales > Moraxellaceae > Acinetobacter > Acinetobacter calcoaceticus/baumannii complex
Accessions
- Primary accessionA3MA34
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000098155 | 1-156 | 6,7-dimethyl-8-ribityllumazine synthase | |||
Sequence: MAIRRIEGLLHLASEGRYAILVGRFNSFVVEHLLEGAIDTLKRHGVNEDNITVIHAPGAWELPIVAKKLATSNQFDAIIALGAVIRGSTPHFDFVAGECAKGLGVVALESSLPVINGVLTTDSIEQAIERSGTKAGNKGSEAALTAIEMVNLLKAI |
Interaction
Subunit
Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.
Structure
Sequence
- Sequence statusComplete
- Length156
- Mass (Da)16,493
- Last updated2008-09-02 v2
- ChecksumAE8164D59C6BE50E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000521 EMBL· GenBank· DDBJ | ABO13778.2 EMBL· GenBank· DDBJ | Genomic DNA |