A2BJU2 · SPEE2_HYPBU
- ProteinPolyamine aminopropyltransferase 2
- GenespeE2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids305 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the biosynthesis of polyamines which are thought to support the growth of thermophilic microorganisms under high-temperature conditions. It seems that long-chain and branched-chain of polyamines effectively stabilize DNA and RNA, respectively. Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to 1,3-diaminopropane to yield sym-norspermidine (bis(3-aminopropyl)amine). It can also synthesize thermospermine from spermidine with a very low activity.
Catalytic activity
- propane-1,3-diamine + S-adenosyl 3-(methylsulfanyl)propylamine = H+ + norspermidine + S-methyl-5'-thioadenosine
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 36 | S-methyl-5'-thioadenosine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 67 | spermidine (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 91 | spermidine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 111 | S-methyl-5'-thioadenosine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 143-144 | S-methyl-5'-thioadenosine (UniProtKB | ChEBI) | ||||
Sequence: DG | ||||||
Active site | 161 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 170 | S-methyl-5'-thioadenosine (UniProtKB | ChEBI) | ||||
Sequence: P |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | spermidine synthase activity | |
Molecular Function | sym-norspermidine synthase activity | |
Molecular Function | thermospermine synthase activity | |
Biological Process | polyamine biosynthetic process | |
Biological Process | spermidine biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePolyamine aminopropyltransferase 2
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Desulfurococcales > Pyrodictiaceae > Hyperthermus
Accessions
- Primary accessionA2BJU2
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000431725 | 1-305 | Polyamine aminopropyltransferase 2 | |||
Sequence: MAQRLPWRFVAEWTSEGEMVLREVKRIYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQCPRKVLVIGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRRYVENCHRKYDAIILDLVDPMEGGPAARLYTLEFYRAVKGCLRPGGAVVTQATSPTLSPRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAASDTVDPAKLSAKEVDELIAARIRGQLRFYDGETHEWMFRLPLPVRQVLSETRDYATDEKPVYVPV |
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 7-242 | PABS | ||||
Sequence: WRFVAEWTSEGEMVLREVKRIYAAGATRYQEYMIAELAGIGKALVLDGKVQSSILDEHWYHEALVHPILLAHQCPRKVLVIGGGEGATVREVLRHSCVEHVTMVDIDEELVELAKKHLAEWHQGAFSSDKLELVIGDGRRYVENCHRKYDAIILDLVDPMEGGPAARLYTLEFYRAVKGCLRPGGAVVTQATSPTLSPRVYAVIRNTLAKVFTIVRPYVSYVRSYNGLWGFVAASD |
Sequence similarities
Belongs to the spermidine/spermine synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length305
- Mass (Da)34,437
- Last updated2007-02-20 v1
- ChecksumA04CBE54D469B5EF
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000493 EMBL· GenBank· DDBJ | ABM80253.1 EMBL· GenBank· DDBJ | Genomic DNA |