A0R109 · OCTT_MYCS2
- ProteinDiglucosylglycerate octanoyltransferase
- GeneoctT
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids252 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Sugar octanoyltransferase likely involved in the biosynthesis of mycobacterial methylglucose lipopolysaccharide (MGLP). Catalyzes the transfer of an octanoyl group from octanoyl-CoA to the C6 OH of the second glucose in diglucosylglycerate (DGG). DGG is the preferred acceptor, but to a lesser extent, GG (glucosylglycerate) can also be used as substrate. DGG and GG are the two earliest intermediates in MGLP biosynthesis.
Catalytic activity
- (2R)-2-O-[alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-glycerate + octanoyl-CoA = (2R)-2-O-[6-O-octanoyl-alpha-D-glucopyranosyl-(1->6)-alpha-D-glucopyranosyl]-glycerate + CoA
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
20 mM | diglucosylglycerate | 37 | ||||
19 mM | glucosylglycerate | 37 | ||||
0.03 mM | octanoyl-CoA | 37 | when DGG is used as cosubstrate | |||
0.02 μM | octanoyl-CoA | 37 | when GG is used as cosubstrate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1939 nmol/min/mg | 37 | with diglucosylglycerate and octanoyl-CoA as substrates | |||
20 nmol/min/mg | 37 | with glucosylglycerate and octanoyl-CoA as substrates |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | O-octanoyltransferase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDiglucosylglycerate octanoyltransferase
- EC number
- Short namesDGG octanoyltransferase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycolicibacterium
Accessions
- Primary accessionA0R109
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000436912 | 1-252 | Diglucosylglycerate octanoyltransferase | |||
Sequence: MSSETSSESTGHRPVLLVFADSLSYFGPTGGLPADDPRIWPNIVGEQLGWDVELIGRIGWTCRDVWWAATQDPRSWAALPRAGAVVFATSGMDSLPSPLPTALREMIRYVRPPWLRRWVRDGYGWVQPRLSPIARSALPPHVTVEYLEMTRNAIDFNRPGIPVVASLPSVHIAETYGRAHHGREPTVRAITAWAEEHHVPLVDLKAAVADEVFGGRGNPDGIHWSFEAHRAVAELMLKGLAEAGVTQRDSAT |
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length252
- Mass (Da)27,762
- Last updated2007-01-09 v1
- Checksum87247413D1175822
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000480 EMBL· GenBank· DDBJ | ABK73636.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001663 EMBL· GenBank· DDBJ | AFP40920.1 EMBL· GenBank· DDBJ | Genomic DNA |