A0QAT1 · PURL_MYCA1

Function

function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site65
Binding site68ATP (UniProtKB | ChEBI)
Binding site112ATP (UniProtKB | ChEBI)
Binding site114Mg2+ 1 (UniProtKB | ChEBI)
Binding site115-118substrate
Active site116Proton acceptor
Binding site137substrate
Binding site138Mg2+ 2 (UniProtKB | ChEBI)
Binding site263substrate
Binding site291Mg2+ 2 (UniProtKB | ChEBI)
Binding site335-337substrate
Binding site523ATP (UniProtKB | ChEBI)
Binding site560ATP (UniProtKB | ChEBI)
Binding site561Mg2+ 1 (UniProtKB | ChEBI)
Binding site563substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylformylglycinamidine synthase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • EC number
  • Short names
    FGAM synthase
  • Alternative names
    • Formylglycinamide ribonucleotide amidotransferase subunit II
      (FGAR amidotransferase II
      ; FGAR-AT II
      )
    • Glutamine amidotransferase PurL
    • Phosphoribosylformylglycinamidine synthase subunit II

Gene names

    • Name
      purL
    • Ordered locus names
      MAV_0744

Organism names

  • Taxonomic identifier
  • Strain
    • 104
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium avium complex (MAC)

Accessions

  • Primary accession
    A0QAT1

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000503231-765Phosphoribosylformylglycinamidine synthase subunit PurL

Interaction

Subunit

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Structure

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias1-17Polar residues
Region1-32Disordered

Sequence similarities

Belongs to the FGAMS family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    765
  • Mass (Da)
    80,662
  • Last updated
    2007-01-09 v1
  • Checksum
    DC672E0FD0E4B834
MTVSPTSAPTQAIDTVERAATTPDEPQPFGELGLKDDEYQRIREILGRRPTDTELAMYSVMWSEHCSYKSSKVHLRYFGETTTDEMRAGMLAGIGENAGVVDIGDGWAVTFKVESHNHPSYVEPYQGAATGVGGIVRDIMAMGARPVAVMDQLRFGAADAPDTRRVLDGVVRGIGGYGNSLGLPNIGGETVFDACYAGNPLVNAMCVGVLRQEDLHLAFASGAGNKIILFGARTGLDGIGGVSVLASDTFDAENSRKKLPSVQVGDPFMEKVLIECCLELYAGGLVIGIQDLGGAGLACATSELASAGDGGMEVRLEAVPLRTAGMTPAEVLCSESQERMCAVVTPENVDAFLAVCRKWDVLATVIGEVTDGDRLRITWHGQTVVDVPPRTVAHEGPVYQRPLARPDTQDALNADSSARLPRPATGAELRATLLALLGSPHLCSRAFITEQYDRYVRGNTVLAEHADGGVLRVDETTGRGIAVSTDASGRYTMLDPYAGAQLALAEAYRNVAVTGATPVAVTNCLNFGSPEDPAVMWQFAQAVRGLADGCVALGIPVTGGNVSFYNQTGSAAILPTPVVGVLGVLDDVSRRLPTALGAEPGETLMLLGETRDEFDGSVWAQVTADHLGGLPPKVDLAREKLLAEVLRAASRDGLVSAAHDLSEGGLAQAVVEAALAGETGCRIVLPEDADPFVTLFSESAGRVLVAVPRTEESRFRSMCEARGLPAVRIGVVDQASDEVEVQGLFTVSLAELRQTSESVLPRLFG

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-17Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000479
EMBL· GenBank· DDBJ
ABK68465.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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