A0A830G7U7 · A0A830G7U7_9EURY
- Protein7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
- GenecofG
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids400 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) from 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil.
Catalytic activity
- 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 5'-deoxyadenosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin + H+ + L-methionine + NH4+
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
Cofactor biosynthesis; coenzyme F0 biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | ammonia-lyase activity | |
Molecular Function | iron ion binding | |
Molecular Function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > Halobacteriaceae
Accessions
- Primary accessionA0A830G7U7
Proteomes
Interaction
Subunit
The FO synthase complex consists of two subunits, CofG and CofH.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 74-310 | Radical SAM core | ||||
Sequence: LSFARNVFLPLTTACRYTCTYCTYYDPPGQATLMTPEEIRETCRRGAAAGCTEALFTFGDDPDDRYTEIHDELADLGHDSIHTYLREACEIALEEGLLPHSNPGDQTYEEMGAVADVNASMGVMLETTAEVRAHGGPRSKVPGQRLNTIANAGKHGIPFTTGILVGLGETWRDRAESLLAIRELDRRYGHVQEVIVQPVTDNERWQEGSPSTATMRRAVAMARAVLPEGVSVQVPPN |
Sequence similarities
Belongs to the radical SAM superfamily. CofG family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length400
- Mass (Da)43,580
- Last updated2021-09-29 v1
- Checksum0A4A5217DA3D6907