A0A0R0BRM3 · A0A0R0BRM3_9GAMM
- Protein2-isopropylmalate synthase
- GeneleuA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids512 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
Catalytic activity
- 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H+
Cofactor
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 2-isopropylmalate synthase activity | |
Molecular Function | acetyl-CoA C-acetyltransferase activity | |
Molecular Function | manganese ion binding | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-isopropylmalate synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Lysobacterales > Lysobacteraceae > Stenotrophomonas
Accessions
- Primary accessionA0A0R0BRM3
- Secondary accessions
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-268 | Pyruvate carboxyltransferase | ||||
Sequence: IRIFDTTLRDGEQSPGCSMSPQQKLVMARALTELGVDIIETGFAASSQSDREALALIAAELKTPVLATLSRCLPGDIETSVKALETAARPRLHVFLSTSPLHREHKLRMSKEQILESVHTHVRLARSYIDDIEFSAEDATRTELDFLTEVAQVAIAAGATTINLPDTVGFTTPEEIRNMFAHVIANAAGSDKVIFSAHCHNDLGLAVANSLAAIEGGARQVEGSINGIGERAGNCAIEEIVMALKVRNAFYGHDTGIDTRRIV | ||||||
Region | 390-512 | Regulatory domain | ||||
Sequence: RLASMTISDVGQRANALVELSDPEGNRVCETASGDGPVDALFAALSAATGVALSLDSYHVHSVGIGADARGEANLSVRHEGEAYEGTGTSRDVIEASALAWLDVANRLLRQRQGSRQAETSNA |
Sequence similarities
Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length512
- Mass (Da)55,363
- Last updated2016-01-20 v1
- Checksum3BB5B472C40B4ABB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LDJH01000022 EMBL· GenBank· DDBJ | KRG55747.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JACIUV010000005 EMBL· GenBank· DDBJ | MBB1117863.1 EMBL· GenBank· DDBJ | Genomic DNA |