A0A7W1BF40 · A0A7W1BF40_9BACT
- ProteinDNA-directed RNA polymerase subunit alpha
- GenerpoA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids351 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | protein dimerization activity | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit alpha
- EC number
- Short namesRNAP subunit alpha
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Gemmatimonadota > Gemmatimonadia > Gemmatimonadales
Accessions
- Primary accessionA0A7W1BF40
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Interaction
Subunit
Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-241 | Alpha N-terminal domain (alpha-NTD) | ||||
Sequence: MTIDLTGLVRPHLVEMTKRDDNPNAAEFRLQPLERGFGYTLGNSLRRLLLSSLRGSAVWGFRLDGVVHEHQTVLGVLEDVHQIVQRLKALTLVVAEDVDDAVLHIRREGAGPVYARDIQEHGAVTLKNPDHLLFTLQDDREVSMELYVNKGRGYVEAEMHPADRSLPVDLVRIDAIYNPVRRANFTVAETRVGQRTDYDRLTVSVETNGTITPEDAIAYAAALAQVHFQYFVDFGKVPTMR | ||||||
Domain | 25-234 | DNA-directed RNA polymerase RpoA/D/Rpb3-type | ||||
Sequence: AAEFRLQPLERGFGYTLGNSLRRLLLSSLRGSAVWGFRLDGVVHEHQTVLGVLEDVHQIVQRLKALTLVVAEDVDDAVLHIRREGAGPVYARDIQEHGAVTLKNPDHLLFTLQDDREVSMELYVNKGRGYVEAEMHPADRSLPVDLVRIDAIYNPVRRANFTVAETRVGQRTDYDRLTVSVETNGTITPEDAIAYAAALAQVHFQYFVDF | ||||||
Region | 255-351 | Alpha C-terminal domain (alpha-CTD) | ||||
Sequence: LRQRLSRPIDDFGLSVRSLNSLKNSNIRTLKDLVEFSEDDLLKVKNVGEKALTEIAELLQREGLNFGMGFEETDGELRILRPGVAPMAQPSAGTGEV |
Domain
The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.
Sequence similarities
Belongs to the RNA polymerase alpha chain family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length351
- Mass (Da)38,834
- Last updated2021-06-02 v1
- Checksum8AED94AF71296C82