A0A7L4CZI4 · A0A7L4CZI4_9AVES
- ProteinProtein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase
- GenePomgnt1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids659 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.
Catalytic activity
- 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl)-L-threonyl-[protein] + UDP + H+
Cofactor
Note: The manganese ion interacts primarily with the substrate UDP-N-acetylglucosamine.
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi membrane | |
Molecular Function | beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity | |
Molecular Function | carbohydrate binding | |
Molecular Function | manganese ion binding | |
Biological Process | O-glycan processing |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase
- EC number
- Short namesPOMGnT1
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Archelosauria > Archosauria > Dinosauria > Saurischia > Theropoda > Coelurosauria > Aves > Neognathae > Neoaves > Telluraves > Coraciimorphae > Coraciiformes > Coraciidae > Eurystomus
Accessions
- Primary accessionA0A7L4CZI4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus membrane ; Single-pass type II membrane protein
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Interacts with DAG1 (via O-linked mannose moiety). Interacts (via transmembrane domain) with FKTN; the interaction is direct and is required for normal location in Golgi membranes.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 123-212 | ILEI/PANDER | ||||
Sequence: RGIHVIVLNQATGHVMAKRVFDTYSPHEDEAMVLFLNMVARGRILIFTVKDEGSFHLKDTAKNVLKSLGSQVAPFLSWRDMWTFVGKKGG |
Domain
The stem domain mediates specific interaction with beta-linked N-acetylglucosamine moieties of O-glycosylated proteins. It also interacts with its product, N-acetyl-beta-D-glucosaminyl-(1->2)-O-alpha-D-mannosylprotein.
Sequence similarities
Belongs to the glycosyltransferase 13 family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusFragment
- Length659
- Mass (Da)75,603
- Last updated2021-04-07 v1
- Checksum7DA5743FA52B79F5
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: M | ||||||
Non-terminal residue | 659 | |||||
Sequence: T |
Keywords
- Technical term