A0A6P1RB25 · A0A6P1RB25_9BRAD
- ProteinThiamine pyrimidine synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids338 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Responsible for the formation of the pyrimidine heterocycle in the thiamine biosynthesis pathway. Catalyzes the formation of hydroxymethylpyrimidine phosphate (HMP-P) from histidine and pyridoxal phosphate (PLP). The protein uses PLP and the active site histidine to form HMP-P, generating an inactive enzyme. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.
Catalytic activity
- N6-(pyridoxal phosphate)-L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + L-histidyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 2 Fe3+ + 4 H2O = L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + (2S)-2-amino-5-hydroxy-4-oxopentanoyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 3-oxopropanoate + 2 Fe2+ + 2 H+This reaction proceeds in the forward direction.
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | metal ion binding | |
Molecular Function | transferase activity | |
Biological Process | thiamine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameThiamine pyrimidine synthase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Bradyrhizobium
Accessions
- Primary accessionA0A6P1RB25
Proteomes
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MLSRRHAFFALSAIALATGSLVTA | ||||||
Chain | PRO_5026723980 | 25-338 | Thiamine pyrimidine synthase | |||
Sequence: APAAELQKASLRLKWLPQAQFAGFYVAAAKGYYKAEGIDLTINPGGPNLLTENLVATGADTFGLSGGTDSVFAARDKGLPIVCIGVSHQITPFIFVTRKDGPVKTIQDFKGKTVTTWFTGANHVLNGMLAKEGIKPDELKIQPQQVSVTPFVDGSVDVVTATYYNEFYTIQSRMPKDSLKTFVAEDYGITFPRDTLIVAEATAKEKPELVKAFLRASLKGWKDAFADPKGAVDTVMAAAPTLDRAQQEFMLTEVKRLMTAGAAAKNGMFWIDADAVKTAHDFFLKYGVISKPLDLAAAYDASFIQSVPLADRLP |
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 43-255 | SsuA/THI5-like | ||||
Sequence: AQFAGFYVAAAKGYYKAEGIDLTINPGGPNLLTENLVATGADTFGLSGGTDSVFAARDKGLPIVCIGVSHQITPFIFVTRKDGPVKTIQDFKGKTVTTWFTGANHVLNGMLAKEGIKPDELKIQPQQVSVTPFVDGSVDVVTATYYNEFYTIQSRMPKDSLKTFVAEDYGITFPRDTLIVAEATAKEKPELVKAFLRASLKGWKDAFADPKGA |
Sequence similarities
Belongs to the NMT1/THI5 family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length338
- Mass (Da)36,481
- Last updated2020-10-07 v1
- ChecksumF723EC435943D0AB