A0A6P0RV05 · A0A6P0RV05_9CYAN
- ProteinOctanoyltransferase
- GenelipB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids216 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.
Miscellaneous
In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.
Catalytic activity
- octanoyl-[ACP] + L-lysyl-[protein] = N6-octanoyl-L-lysyl-[protein] + holo-[ACP] + H+
Pathway
Protein modification; protein lipoylation via endogenous pathway; protein N6-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 77-84 | substrate | ||||
Sequence: RGGEVTYH | ||||||
Site | 141 | Lowers pKa of active site Cys | ||||
Sequence: K | ||||||
Binding site | 144-146 | substrate | ||||
Sequence: AIG | ||||||
Binding site | 157-159 | substrate | ||||
Sequence: GFA | ||||||
Active site | 175 | Acyl-thioester intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | ribonuclease MRP complex | |
Molecular Function | lipoyl(octanoyl) transferase activity | |
Biological Process | protein lipoylation | |
Biological Process | rRNA processing | |
Biological Process | tRNA processing |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameOctanoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Oscillatoriophycideae > Oscillatoriales > Microcoleaceae > Symploca
Accessions
- Primary accessionA0A6P0RV05
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 35-213 | BPL/LPL catalytic | ||||
Sequence: PSLDDVLLLLEHPAVYTLGRGASLEFLKFDPNVVDFPTYRVERGGEVTYHCPGQLVGYPILNLRYYQQDLHWYLRQLEEVLIQLLGIYGLKGERITGMTGVWLEGRKVAAIGIKASRWITMHGFALNVCPELSGFEKIVPCGIANKPVGSLAQFIPGIRVEQVRHLVATKFAQVFNVEL |
Sequence similarities
Belongs to the LipB family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length216
- Mass (Da)24,482
- Last updated2020-10-07 v1
- Checksum1DA6427D9212A45B