A0A662PK44 · A0A662PK44_UNCTR
- ProteintRNA carboxymethyluridine synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids519 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
Catalytic activity
- acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine34 in tRNA = 5'-deoxyadenosine + 5-(carboxymethyl)uridine34 in tRNA + CoA + 2 H+ + L-methionineThis reaction proceeds in the forward direction.
CHEBI:57288 + CHEBI:15377 + CHEBI:59789 + RHEA-COMP:11727 CHEBI:65315 Position: 34= CHEBI:17319 + RHEA-COMP:10407 CHEBI:74882 Position: 34+ CHEBI:57287 + 2 CHEBI:15378 + CHEBI:57844
Cofactor
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
tRNA modification.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | acyltransferase activity | |
Molecular Function | iron-sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | tRNA binding | |
Biological Process | tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA carboxymethyluridine synthase
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageArchaea > Euryarchaeota > Thermococci
Accessions
- Primary accessionA0A662PK44
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 65-348 | Radical SAM core | ||||
Sequence: RTISGIAVIAVMTSPYPCPHGKCSYCPGGPERGVPQSYTGLEPATMRAIQNEYHPRKQVEHRINQLRKIGHPVEKAEVIIMGGTFTAMPYDYQKWFVKEILDGLTGKTTSSIEEAQKIAEKSEIRNTGLTVETRPDWAKEDQIDRILSLGATRIELGIQSIYEDVLERVNRGHTVEDIVEATRIARDSGFKICYHIMPGLPGSDLKRDYKMFERIFRDSQFKPDMIKIYPCLVIEGTKLYDEWKRGEYTPIRDKEAIELISKVKGELIPKWVRTMRIQRDIPVK |
Sequence similarities
Belongs to the ELP3 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length519
- Mass (Da)60,258
- Last updated2020-04-22 v1
- Checksum211F5BD60D89724A