A0A660RFA2 · A0A660RFA2_9THEM
- ProteinPyridoxal 5'-phosphate synthase subunit PdxT
- GenepdxT
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids199 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.
Catalytic activity
- H2O + L-glutamine = L-glutamate + NH4+
Pathway
Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 46-48 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: GES | ||||||
Active site | 78 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 105 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 134-135 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: IR | ||||||
Active site | 170 | |||||
Sequence: H | ||||||
Active site | 170 | Charge relay system | ||||
Sequence: H | ||||||
Active site | 172 | |||||
Sequence: E | ||||||
Active site | 172 | Charge relay system | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | glutaminase complex | |
Molecular Function | glutaminase activity | |
Molecular Function | pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity | |
Biological Process | glutamine catabolic process | |
Biological Process | pyridoxal phosphate biosynthetic process | |
Biological Process | pyridoxine metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyridoxal 5'-phosphate synthase subunit PdxT
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Thermotogota > Thermotogae > Thermotogales > Thermotogaceae > Thermotoga
Accessions
- Primary accessionA0A660RFA2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.
Structure
Sequence
- Sequence statusComplete
- Length199
- Mass (Da)22,101
- Last updated2020-04-22 v1
- Checksum7EAA7769292E462E