A0A520W1Z8 · A0A520W1Z8_9ACTN
- ProteinNADH-quinone oxidoreductase subunit B
- GenenuoB
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids153 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity
- a quinone + NADH + 5 H+(in) = a quinol + NAD+ + 4 H+(out)
CHEBI:132124 + CHEBI:57945 + 5 H+ (in)CHEBI:15378= CHEBI:24646 + CHEBI:57540 + 4 H+ (out)CHEBI:15378
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | iron ion binding | |
Molecular Function | NADH dehydrogenase (ubiquinone) activity | |
Molecular Function | NADH:ubiquinone reductase (non-electrogenic) activity | |
Molecular Function | quinone binding | |
Biological Process | aerobic respiration | |
Biological Process | electron transport coupled proton transport |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADH-quinone oxidoreductase subunit B
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Candidatus Actinomarinidae > Candidatus Actinomarinales
Accessions
- Primary accessionA0A520W1Z8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Interaction
Subunit
NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 33-141 | NADH:ubiquinone oxidoreductase-like 20kDa subunit | ||||
Sequence: CCAIEMMAAGSAKYDLARYGMEVFRASPRQADLMIVAGRVSQKMAPVLRQVYDQMADPKWVIAMGACSSTGGMFNNYALVQGVDHIVPVDIYVPGCPPGPQSLMHGILT |
Sequence similarities
Belongs to the complex I 20 kDa subunit family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length153
- Mass (Da)16,878
- Last updated2019-10-16 v1
- Checksum75F8D7F2C9697B4B