A0A4Y9RXB1 · A0A4Y9RXB1_9CAUL

  • Protein
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • Gene
    purL
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site52
Binding site55ATP (UniProtKB | ChEBI)
Binding site94ATP (UniProtKB | ChEBI)
Binding site96Mg2+ 1 (UniProtKB | ChEBI)
Binding site97-100substrate
Active site98Proton acceptor
Binding site119substrate
Binding site120Mg2+ 2 (UniProtKB | ChEBI)
Binding site243substrate
Binding site271Mg2+ 2 (UniProtKB | ChEBI)
Binding site315-317substrate
Binding site498ATP (UniProtKB | ChEBI)
Binding site535ATP (UniProtKB | ChEBI)
Binding site536Mg2+ 1 (UniProtKB | ChEBI)
Binding site538substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular Functionphosphoribosylformylglycinamidine synthase activity
Biological Process'de novo' IMP biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphoribosylformylglycinamidine synthase subunit PurL
  • EC number
  • Short names
    FGAM synthase
  • Alternative names
    • Formylglycinamide ribonucleotide amidotransferase subunit II
      (FGAR amidotransferase II
      ; FGAR-AT II
      )
    • Glutamine amidotransferase PurL
    • Phosphoribosylformylglycinamidine synthase subunit II

Gene names

    • Name
      purL
    • ORF names
      EGY25_10515

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • B-10
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Caulobacterales > Caulobacteraceae > Brevundimonas

Accessions

  • Primary accession
    A0A4Y9RXB1

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain16-56Phosphoribosylformylglycinamidine synthase linker
Domain77-191PurM-like N-terminal
Domain205-358PurM-like C-terminal
Domain441-559PurM-like N-terminal
Domain574-707PurM-like C-terminal

Sequence similarities

Belongs to the FGAMS family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    738
  • Mass (Da)
    78,365
  • Last updated
    2019-09-18 v1
  • Checksum
    87F48F5A48142A27
MSAPQKTPEKSMAELAAEYGLAPNEYQVVLDRLGREPNHVELGVFSVMWSEHCSYKSSKIHLGKFPTTGDRVICGPGENAGVIDIDDGDACIFKMESHNHPSYIEPYQGAATGVGGIMRDVFTMGARPVALLNALRFGDPSHEKTKRLVKGVVSGIGGYGNCVGVPTVAGETNFHRGYNGNILVNAMCVGLAKSDSIFYSAAPSAGLSVVYFGSKTGRDGIHGATMSSAEFDDESESKRPTVQVGDPFAEKLLIEATLELMASGAVAAIQDMGAAGLTSSSVEMAGKGGVGIELNMDAVPQREEGMSAYEMMLSESQERMLAVLKPGREADGERIFKKWGLDAATIGVTTDTGRLVLKHHGETVCDVPLAPLFDDAPLYDRPWVQPELHPRLNPADIPAPENWADAVMKVIACPDMASKRWIWEQYDRHVMADTLQDSATGADAGVVRVHGTDKGLAVTSDCTPRYVQNDPYEGGKQAVAEAWRNLTAVGSRPIAITDNLNFGNPQRPEIMGQIVRAIDGMAEACRELVFPVVSGNVSLYNETNGVAIPPTPTVGAVGLLPNYDVVTNFSTMAEGDTLVLIGQTVGELGASIYLREVLGREDGAPPPVDLKLEKKTGDFVRDLIEAGDLTVVHDLSDGGLIGAAADLALASDVGVTLDASSAAHAHIFLFGEDQARYLVAVPDADALIAKAREAGLHASVVGRAGGDAFGSTDLFSIPVAHLREWHEGWMPGWLGDAA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
SPVH01000006
EMBL· GenBank· DDBJ
TFW12435.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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