A0A3A9ZCN3 · A0A3A9ZCN3_9ACTN

  • Protein
    Glutamine-dependent NAD(+) synthetase
  • Gene
    nadE
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site72Proton acceptor
Active site72Proton acceptor; for glutaminase activity
Active site154For glutaminase activity
Binding site160L-glutamine (UniProtKB | ChEBI)
Active site190Nucleophile; for glutaminase activity
Binding site217L-glutamine (UniProtKB | ChEBI)
Binding site223L-glutamine (UniProtKB | ChEBI)
Binding site359-366ATP (UniProtKB | ChEBI)
Binding site436deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site465deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site581deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Molecular Functionnitrilase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutamine-dependent NAD(+) synthetase
  • EC number
  • Alternative names
    • NAD(+) synthase [glutamine-hydrolyzing]

Gene names

    • Name
      nadE
    • ORF names
      D7294_05775

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • KCTC 29097
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Kitasatosporales > Streptomycetaceae > Streptomyces

Accessions

  • Primary accession
    A0A3A9ZCN3

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain32-287CN hydrolase
Region504-531Disordered

Sequence similarities

Belongs to the NAD synthetase family.
In the C-terminal section; belongs to the NAD synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    615
  • Mass (Da)
    66,634
  • Last updated
    2018-12-05 v1
  • Checksum
    E8C0A6BACFFD64B7
MARERRPPPGPRGRRRPHCVNRTIRLGGVPQLRLALNQIDSTVGDLPGNADAVLRWTRHGVERGAHLVVFPEMMLTGYPVEDLALRSSFVSAAREALRGLAARLAAEGLGETPVLLGYLDRAEAVPERPWQPVGTPQNAAAVLHRGRVVLSFAKHHLPNYGVFDEFRYFVPGETLPVVRVHGVDVALAICEDLWQDGGRVPAARAAGAGLLVAINASPYEVAKEDVRLDLVRKRAQEAGCTLAYLAMTGGQDELVYDGDSLVVDRNGEVLLRGPQFEEECLLVDLELPPAAAEPPGGVVEDGLRIDRVTLSEDPLPPYEPAYPGGLAPALDDAEVRYRALVTGLRAYVRKNGFGSVLIGLSGGVDSALCAVLACDALGAEHVFALSMPSAYSSEHSRTDAEELAARTGLTLRTVPIAPMFDAYMGAVGLTGLAEENLQSRLRGTLLMAVSNQEGHIVLAPGNKSELAVGYSTLYGDSVGAYGPIKDLYKTGVYQLARWRNRAAAERGETPPIPENTLVKPPSAELRPGQVDTDSLPDYDVLDRVLELYVDRDTGREGIIAAGFDPELVTRVLRLVDTAEYKRRQYPPGTKISPKVFGKDRRLPLTNRWREDGLPT

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
RBAL01000002
EMBL· GenBank· DDBJ
RKN45935.1
EMBL· GenBank· DDBJ
Genomic DNA

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