A0A349H5N2 · A0A349H5N2_9BACT

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

TypeIDPosition(s)Description
Site36Interaction with DNA
Site141Interaction with DNA
Site142Interaction with DNA
Site145Interaction with DNA
Site150Interaction with DNA
Site157Interaction with DNA
Active site286O-(5'-phospho-DNA)-tyrosine intermediate
Site288Interaction with DNA
Site471Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      DCS23_00765

Organism names

Accessions

  • Primary accession
    A0A349H5N2

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain6-117Toprim
Region165-170Interaction with DNA

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    741
  • Mass (Da)
    82,696
  • Last updated
    2018-11-07 v1
  • Checksum
    049E152844AECFF1
MTVKQYKLLIVESPSKAKTIGKYLGDDYIVKASVGHVRDLPKSNKQAIDIPGGFIPHYEISKGKAEVLSEIDSLAKKASEVLLATDPDREGEAIAWHIAEACKLKNPKRVTYHEITKTAIEEAIAHPRKIDQNLRKAQEARRVLDRLVGYDLSGLIWKKVRYGLSAGRVQSPALRIIMEREREIRAFIPEAYWSLTAKVKTEKGFELELSCTEEPRDEKIGKDILKIGRSNQWIVREVKESEVGRAPKAPFITSTLQQAGSSRFGFAPSRTMGIAQKLYEKGFITYMRTDSTTLSADAQKAVIAEATKRYGASSAEARVYKTKSKNAQEAHEAIRPTDMSKDNLGINPEEKKLYKLIWARTMASQMVDAKLARTTILANVRDFSIPNFSVTGSRVIMPGWLSADPDSRGEDVELPKVAEGESLILLSLNDEAKFTEPPSRYSEAGLVKELEKRDIGRPSTYASIIKTICDRGYVIKDGKTLKPTDTGDVVSSFLEEHFANYISDTFTAEMENKLDDIANGEREYVKVLKEFYVPFTKDLKAKDEIEKQTNMGDADPKHKCPKCNGPMIIKLARNGKFLSCKKYPDCEGARTIDGEELEGPRDTGELCPKCEKANLVERDGRFGRFIACGSYPKCKFIKKDETAALLNHTGVGCPVCKKGFMTERKGRFGIFYSCTGYPDCKYAIKAKPTGKICQYPKVDGTPCGSLMMDGTKTIPERCSDKTCPNHNPHKLEKARIDAKGK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DMXP01000002
EMBL· GenBank· DDBJ
HAS84595.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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