A0A317C3I8 · A0A317C3I8_9GAMM

Function

function

Degrades single-stranded DNA (ssDNA) in a highly processive manner. Also functions as a DNA deoxyribophosphodiesterase that releases deoxyribose-phosphate moieties following the cleavage of DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease or AP lyase.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
    EC:3.1.11.1 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+ ions per monomer.

Features

Showing features for binding site.

147550100150200250300350400450
TypeIDPosition(s)Description
Binding site10Mg2+ 1 (UniProtKB | ChEBI)
Binding site12Mg2+ 2 (UniProtKB | ChEBI)
Binding site12substrate
Binding site160substrate
Binding site181Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular Functionmetal ion binding
Molecular Functionsingle-stranded DNA 3'-5' DNA exonuclease activity
Biological ProcessDNA repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Exodeoxyribonuclease I
  • EC number

Gene names

    • ORF names
      DKW60_19030

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • JCM 18388
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Thiotrichales > Thiotrichaceae > Leucothrix

Accessions

  • Primary accession
    A0A317C3I8

Proteomes

Interaction

Subunit

Monomer. Interacts with ssb (via C-terminus); this interaction stimulates the exonuclease activity by recruiting the enzyme to its substrate.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain197-351ExoI SH3-like
Domain354-475ExoI C-terminal

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    475
  • Mass (Da)
    54,407
  • Last updated
    2018-10-10 v1
  • Checksum
    F6636956A2141E66
MAKTTLFWHDYETWGINPRKDRASQFAGIRTNLELEIIDEPVMLYCQPGPDFIPHPEAVLVTGITPQKAAAEGVSEADFFHRVNEQLSKPGTCGVGYNSIRFDDEVSRFGFYRNFIDPYAREWQNGNSRWDLLDLVRMTHALRPDGIEWPEREPGIPSFRLEHLTAANNIEHEGAHDALVDVRATIAIAKLIKEKQPRLYEFYFALRQKNKAAELLNLEKQKTVLHISGMIPASMGCVSPIVPLMQHPVNSNEFICFDLRQHPQQLLNASVEEIEYSLYTPNAERDDDSPRIALKGVHINKCPALAPTNTLTPELAEKWQINWDEIEQHKQLLLADSGLKAKLKALYSAPRDHGPVDADSALYNGFINNEDRKLCNFVINQSAERLAHENIAFSDPRLQTLLFRYRARNWPETLDQQEQWKWQEFCRARLIDGEFGCEFTANDFQDVMQDIAQRDLSDGQKVALQGLLDWVQGLQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
QGKM01000075
EMBL· GenBank· DDBJ
PWQ92839.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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