A0A2U8GKL6 · A0A2U8GKL6_9RHOO
- ProteinCarbamoyltransferase HypF
- GenehypF
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids786 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Involved in the maturation of [NiFe] hydrogenases. Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases. HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide.
Catalytic activity
- ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + H+ + phosphate
Pathway
Protein modification; [NiFe] hydrogenase maturation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 27 | |||||
Sequence: R | ||||||
Active site | 45 | |||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acylphosphatase activity | |
Molecular Function | carboxyl- or carbamoyltransferase activity | |
Molecular Function | double-stranded RNA binding | |
Molecular Function | ligase activity | |
Molecular Function | zinc ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarbamoyltransferase HypF
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Rhodocyclales > Zoogloeaceae > Parazoarcus
Accessions
- Primary accessionA0A2U8GKL6
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 12-99 | Acylphosphatase-like | ||||
Sequence: RRHIRVRGVVQGVGFRPFVYRLAQTLDLSGWVRNDGDGVELEGQGLPGNLSALIARIRSEAPSLARVDSIHTRLCDADPADQGFTIRT | ||||||
Domain | 208-415 | YrdC-like | ||||
Sequence: RDPIADTLLRLLTGEIVAIKGLGGFHLVCDARNPEAVERLRARKGRGGKPFAVMVANLASARRWARLASAEEALLAGAERPVVLCEKRPSVDNELWGLAPDLGSIGLMLPYTPLHYLLFHDNAGRPSGTDWLGEVQDLALVMTSANPGGEPLVTDNQEALQRLKGIADAYLMHDRDILSRCDDSVMVAERSALAGARETPRFVRRARG |
Sequence similarities
Belongs to the carbamoyltransferase HypF family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length786
- Mass (Da)84,654
- Last updated2018-09-12 v1
- ChecksumB2D969FB96ADE76E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP022187 EMBL· GenBank· DDBJ | AWI73930.1 EMBL· GenBank· DDBJ | Genomic DNA |