A0A2R6PI27 · LDOX_ACTCC
- ProteinAnthocyanin synthase
- GeneLDOX
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids355 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in anthocyanin biosynthesis by catalyzing the oxidation of leucoanthocyanidins into anthocyanidins (PubMed:30912865).
Required for the accumulation of anthocyanin in red-fleshed kiwifruit varieties (PubMed:30912865).
Required for the accumulation of anthocyanin in red-fleshed kiwifruit varieties (PubMed:30912865).
Catalytic activity
- 2-oxoglutarate + a (2R,3S,4S)-leucoanthocyanidin + O2 = a 4-H-anthocyanidin with a 3-hydroxy group + CO2 + 2 H2O + succinateThis reaction proceeds in the forward direction.
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 ascorbate molecule per subunit.
Note: Binds 1 Fe2+ ion per subunit.
Pathway
Pigment biosynthesis; anthocyanin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 145 | substrate | ||||
Sequence: Y | ||||||
Binding site | 216 | substrate | ||||
Sequence: K | ||||||
Binding site | 218-220 | 2-oxoglutarate (UniProtKB | ChEBI) | ||||
Sequence: NYY | ||||||
Binding site | 235 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 236 | substrate | ||||
Sequence: T | ||||||
Binding site | 237 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 291 | Fe cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 301 | 2-oxoglutarate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 301-303 | 2-oxoglutarate (UniProtKB | ChEBI) | ||||
Sequence: RIS | ||||||
Binding site | 309 | substrate | ||||
Sequence: E | ||||||
Binding site | 344 | substrate | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | L-ascorbic acid binding | |
Molecular Function | leucocyanidin oxygenase activity | |
Molecular Function | metal ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, with 2-oxoglutarate as one donor, and the other dehydrogenated | |
Biological Process | anthocyanin-containing compound biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAnthocyanin synthase
- EC number
- Short namesAcANS
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > Ericales > Actinidiaceae > Actinidia
Accessions
- Primary accessionA0A2R6PI27
- Secondary accessions
Proteomes
Genome annotation databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000448077 | 1-355 | Anthocyanin synthase | |||
Sequence: MVATVVGTRVESLASSGIEAIPKEYVRPQEELTSIGNIFEEEKKENGPQVPTIDLEDLVSEDEEKRVRCHEELKRAATEWGVMQVVNHGIPIELMERVRAAGAEFFNQSVEEKEKYANDHASGNIQGYGSKLANNASGQLEWEDYFFHLVYPEDKRDMSIWPKTPSDYIPATSAYAEHLRGLATKILSALSLGLGLEEGRLEKEVGGMEELLLQMKINYYPKCPQPELALGVEAHTDVSALTFILHNMVPGLQLFYEGKWVTAKCVPDSLVMHIGDTIEILSNGKYKSILHRGLVNKEKVRISWAVFCEPPKEKIILKPLPETVSEAEPPLYPPRTFAQHIHHKLFRKTQELGAK |
Expression
Tissue specificity
Expressed in stems and leaves (PubMed:25143057).
Expressed at low levels in ovaries (PubMed:21175894, PubMed:25143057).
Expressed at low levels in ovaries (PubMed:21175894, PubMed:25143057).
Developmental stage
Expressed in the inner and outer pericarps of developing fruit from 20 to 49 days after flowering (DAF), and expression decreases as the fruit ripens.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 211-310 | Fe2OG dioxygenase | ||||
Sequence: LLLQMKINYYPKCPQPELALGVEAHTDVSALTFILHNMVPGLQLFYEGKWVTAKCVPDSLVMHIGDTIEILSNGKYKSILHRGLVNKEKVRISWAVFCEP |
Sequence similarities
Belongs to the iron/ascorbate-dependent oxidoreductase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length355
- Mass (Da)39,959
- Last updated2018-06-20 v1
- Checksum3153D1F58830B37B
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 42 | in Ref. 1; AGV53047 | ||||
Sequence: E → G | ||||||
Sequence conflict | 60 | in Ref. 1; AGV53047 | ||||
Sequence: S → P | ||||||
Sequence conflict | 201 | in Ref. 1; AGV53047 | ||||
Sequence: L → P |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KF157392 EMBL· GenBank· DDBJ | AGV53047.1 EMBL· GenBank· DDBJ | mRNA | ||
NKQK01000025 EMBL· GenBank· DDBJ | PSR91531.1 EMBL· GenBank· DDBJ | Genomic DNA |