A0A2M6VP28 · A0A2M6VP28_9BURK

Function

function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N6-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site78[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site83[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site89[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site104[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site108[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site111[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site318[4Fe-4S] cluster 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionlipoate synthase activity
Molecular Functionmetal ion binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lipoyl synthase
  • EC number
  • Alternative names
    • Lip-syn
      (LS
      )
    • Lipoate synthase
    • Lipoic acid synthase
    • Sulfur insertion protein LipA

Gene names

    • Name
      lipA
    • ORF names
      B9Z40_14570

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 15K
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Limnohabitans

Accessions

  • Primary accession
    A0A2M6VP28

Proteomes

Subcellular Location

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain89-307Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    335
  • Mass (Da)
    37,522
  • Last updated
    2018-04-25 v1
  • Checksum
    09E93BBE37717ACC
MSNTPTERTVVREVQSVENYDPMAKQKAAAKLSRIPVKVEAAEVLKKPDWIRVKAASPTTRFYEIKDVLRANKLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPDNLAKTIAQLKLQYVVITSVDRDDLRDGGAGHFVECIRKTRELSPTTQIEVLVPDFRGRDDRALEILKAAPPDVMNHNMETVPRLYKEARPGSDYQFSLNLLKKFKALFPNVPTKSGLMVGLGETDEEIIEVMRDMRAHNIEMLTIGQYLAPSNSHLPVRRYVHPDTFKMFEAKAYEMGFSHAAVGAMVRSSYHADQQAHSAQINVKGL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NESM01000005
EMBL· GenBank· DDBJ
PIT80200.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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