A0A292Z1H4 · A0A292Z1H4_9PSEU

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site46-50NAD+ (UniProtKB | ChEBI)
Binding site95-96NAD+ (UniProtKB | ChEBI)
Binding site125NAD+ (UniProtKB | ChEBI)
Active site127N6-AMP-lysine intermediate
Binding site148NAD+ (UniProtKB | ChEBI)
Binding site188NAD+ (UniProtKB | ChEBI)
Binding site304NAD+ (UniProtKB | ChEBI)
Binding site328NAD+ (UniProtKB | ChEBI)
Binding site422Zn2+ (UniProtKB | ChEBI)
Binding site425Zn2+ (UniProtKB | ChEBI)
Binding site441Zn2+ (UniProtKB | ChEBI)
Binding site447Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological Processbase-excision repair, DNA ligation
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      TOK_0694

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • N23
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Pseudonocardia

Accessions

  • Primary accession
    A0A292Z1H4

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-20Disordered
Region570-631Disordered
Compositional bias582-615Acidic residues
Domain709-781BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    802
  • Mass (Da)
    85,794
  • Last updated
    2017-12-20 v1
  • Checksum
    BD2EF7A684395D02
MSTDSPAGTAPRPAPDADRERHAALATEIADHQFRYYVLDAPVLSDGQFDELWRELVGLEERYPDLITADSPTQKVGSRFATEFVAHDHLERMLSLDNAFSADELRGWAERVGREVGADAHYLCELKIDGLAVNLLYENGKLVRALTRGDGRTGEDVTLNMRTLAEVPEMLTGTAEFPVPELVEVRGEVYFRLEDFQALNASLVEAGKPPFANPRNTAAGSLRQKDPRVTGSRNLRLICHGLGKREGFNPERLSQAYDALRTWGLPVSERTKIVQGIDDVVAHVEYWGEHRHDIEHEIDGVVVKVDEVALQRRLGATSRAPRWAIAFKYPPEEVTTKLLDIQVNVGRTGRVTPFAFMEPVVVAGSTVSLATLHNADEVRRKGVLIGDRVVIRKAGDVIPEVLGPIIDVRDGTERAFVMPTHCPECGTELAQQKAGDVDIRCPNARSCPAQLRERLFHIAGRGAFDIEALGYKAATALLEAGVVTDEGDVFSLTEEDLLQVDLFRTKAGVLSANGRKLLDNLEKAKDRPLWRVLVGLSIRHVGPTAAQALAREFGSLEAIEEAAARAAEETAKAGVEITSDGSSDEPDADFVPDVDSVPDAVEEPDAVEEPDAVEEPAAAGDSGAGDSGADAGADAAEAAAVAAAKAERDATRARAKAVAAALAPIAGGEGVGPTIAAALRDWFTVDWHRDVVAKWRAAGVRTVDEVDASIPRTLDGLSIVITGSMAGWSRDEAKEAIAARGGRAAGSVSKKTAFLVAGDAPGSKYDKALELGVPILDEAGLGVLLASGPEEAEKVATLAPTD

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias582-615Acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BEGX01000114
EMBL· GenBank· DDBJ
GAY12300.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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