A0A257E3B1 · A0A257E3B1_9PSED
- ProteinATP-dependent dethiobiotin synthetase BioD
- GenebioD
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids226 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
Catalytic activity
- (7R,8S)-7,8-diammoniononanoate + CO2 + ATP = (4R,5S)-dethiobiotin + ADP + phosphate + 3 H+
Cofactor
Pathway
Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 13-18 | ATP (UniProtKB | ChEBI) | ||||
Sequence: DVGKTT | ||||||
Binding site | 17 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Active site | 38 | |||||
Sequence: K | ||||||
Binding site | 42 | substrate | ||||
Sequence: S | ||||||
Binding site | 55 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 55 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 117 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 117-120 | ATP (UniProtKB | ChEBI) | ||||
Sequence: EGAG | ||||||
Binding site | 206-208 | ATP (UniProtKB | ChEBI) | ||||
Sequence: PKL |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | dethiobiotin synthase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | biotin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent dethiobiotin synthetase BioD
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionA0A257E3B1
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length226
- Mass (Da)23,427
- Last updated2017-12-20 v1
- ChecksumA59021457A61350D