A0A210VWS8 · A0A210VWS8_9BURK
- ProteinHPr kinase/phosphorylase
- GenehprK
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids318 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr).
Miscellaneous
Both phosphorylation and phosphorolysis are carried out by the same active site and suggest a common mechanism for both reactions.
Catalytic activity
- [HPr protein]-L-serine + ATP = [HPr protein]-O-phospho-L-serine + ADP + H+
- [HPr protein]-O-phospho-L-serine + H+ + phosphate = [HPr protein]-L-serine + diphosphate
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 146 | |||||
Sequence: H | ||||||
Binding site | 161-168 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GESGLGKS | ||||||
Active site | 167 | |||||
Sequence: K | ||||||
Binding site | 168 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 185 | Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity | ||||
Sequence: D | ||||||
Binding site | 210 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 252 | |||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | phosphorelay sensor kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Molecular Function | protein serine/threonine/tyrosine kinase activity | |
Biological Process | regulation of carbohydrate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHPr kinase/phosphorylase
- EC number
- Short namesHPrK/P
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Delftia
Accessions
- Primary accessionA0A210VWS8
Proteomes
Interaction
Subunit
Homohexamer.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 11-135 | HPr(Ser) kinase/phosphorylase N-terminal | ||||
Sequence: LFEEFRSSLRWEWVAGLGASERRFDEMAVRSARSGADLVGYLNYIHPYRLQVLGEREIAYLSNATPQDCQRRVARIVTLEPPVLVLADGQTAPDALLSMCERAHIPMFSTREQSAFVIDVLRAYL | ||||||
Domain | 138-307 | HPr kinase/phosphorylase C-terminal | ||||
Sequence: HFADRTTMHGVFMDILGMGVLITGESGLGKSELGLELVTRGNGLVADDAVDLYRINQTTIEGKCPELLQNLLEVRGIGLLDIRAIFGETAVRRKMRLKLIVHLVRKETMEREYERLPAEPLTQDVLGVPVRKVVIQVVAGRNIAVLVEAAVRNAILQLRGIDTYQEFVQR | ||||||
Region | 209-218 | Important for the catalytic mechanism of both phosphorylation and dephosphorylation | ||||
Sequence: LEVRGIGLLD | ||||||
Region | 273-278 | Important for the catalytic mechanism of dephosphorylation | ||||
Sequence: QVVAGR |
Domain
The Walker A ATP-binding motif also binds Pi and PPi.
Sequence similarities
Belongs to the HPrK/P family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length318
- Mass (Da)35,725
- Last updated2017-11-22 v1
- Checksum8203286873619C91