A0A1U2BW66 · A0A1U2BW66_9MYCO
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids356 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine34 in tRNA = 2-thiouridine34 in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein]
CHEBI:17499 + CHEBI:30616 + RHEA-COMP:11726 + RHEA-COMP:11727 CHEBI:65315 Position: 34= RHEA-COMP:11728 CHEBI:87170 Position: 34+ CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378 + RHEA-COMP:10131
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 6-13 | ATP (UniProtKB | ChEBI) | ||||
Sequence: AMSGGVDS | ||||||
Binding site | 32 | ATP (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Active site | 101 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 125 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Site | 126 | Interaction with tRNA | ||||
Sequence: H | ||||||
Active site | 193 | Cysteine persulfide intermediate | ||||
Sequence: C | ||||||
Site | 333 | Interaction with tRNA | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | methyltransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | methylation | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacteroides > Mycobacteroides abscessus
Accessions
- Primary accessionA0A1U2BW66
Proteomes
Subcellular Location
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 143-145 | Interaction with tRNA | ||||
Sequence: KDQ | ||||||
Domain | 202-267 | tRNA-specific 2-thiouridylase MnmA-like central | ||||
Sequence: AFLGARIGVRRGNVVDADGSVLATHAGVHEFTIGQRKGLGLVGPAADGRPRYVTSIDAETATVRVG | ||||||
Domain | 280-353 | tRNA-specific 2-thiouridylase MnmA-like C-terminal | ||||
Sequence: EPVVWTSGRVPVQPIECQVQVRAHGSVVDAVVEPGAERIHVRLRTALRGVAPGQTVVLYRPDAEGDEVLGSAVI |
Sequence similarities
Belongs to the MnmA/TRMU family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length356
- Mass (Da)37,918
- Last updated2017-05-10 v1
- Checksum21BD9DA69AEFC7FB