A0A1Q7TBE9 · A0A1Q7TBE9_9BACT
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids342 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- uridine34 in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 2-thiouridine34 in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H+
RHEA-COMP:11727 CHEBI:65315 Position: 34+ RHEA-COMP:11726 + CHEBI:17499 + CHEBI:30616 = RHEA-COMP:11728 CHEBI:87170 Position: 34+ RHEA-COMP:10131 + CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378
Features
Showing features for binding site, active site, site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Taxonomic lineageBacteria > Verrucomicrobiota
Accessions
- Primary accessionA0A1Q7TBE9
Proteomes
Subcellular Location
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 148-150 | Interaction with tRNA | ||||
Sequence: KDQ | ||||||
Domain | 186-240 | tRNA-specific 2-thiouridylase MnmA-like central | ||||
Sequence: FHPGGIYDLDGNFIAGHDGIEMFTIGQRKGLPGGSAQPRYVVDLDPETNRVIVGQ | ||||||
Domain | 248-333 | tRNA-specific 2-thiouridylase MnmA-like C-terminal | ||||
Sequence: EFEIDRVNWISGAGNDDPGPRSATAATMAHEVTVKIRYNHPGTRATLMPMENQRARIRLHDPQKAVTPGQAAVFYDDDLVLGGGWI | ||||||
Region | 284-285 | Interaction with tRNA | ||||
Sequence: RY |
Sequence similarities
Belongs to the MnmA/TRMU family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length342
- Mass (Da)37,754
- Last updated2017-04-12 v1
- Checksum6219A78B4CA09053